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siRNA Id: | aspsirna0195
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siRNA Name: | siT11/C12
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| Gene: | Amyloid beta (A4) precursor protein(APP) |
| Disease: | Alzheimers disease |
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Wild allele/target (5'->3'): | CUGAAGUGAAGAUGGAUGCA
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Mutant allele/target (5'->3'): | CUGAAGUGAAUCUGGAUGCA
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Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
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Wild siRNA (As strand): | GACUUCACUUCUACCUACGU
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ASP-siRNA (As strand): | GACUUCACUUAGACCUACGU
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Mismatch position in siRNA: | A10C,G9U |
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Mutant allele (5'->3'): | CUGAAGUGAAUCUGGAUGCA
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ASP-siRNA (As strand): | GACUUCACUUAGACCUACGU
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ASP-siRNA efficacy for mutant allele: | 45
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Wild allele (5'->3'): | CUGAAGUGAAGAUGGAUGCA
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ASP-siRNA (As strand): | GACUUCACUUAGACCUACGU
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ASP-siRNA efficacy for wild allele: | Poor efficacy
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Percentage discrimination for ASP-siRNA: | Moderate discrimination
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Wild allele (5'->3'): | CUGAAGUGAAGAUGGAUGCA
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Wild siRNA (As strand): | GACUUCACUUCUACCUACGU
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Wild sirna efficacy for wild allele: | High efficacy
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Wild allele (5'->3'): | CUGAAGUGAAGAUGGAUGCA
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ASP-siRNA (As strand): | GACUUCACUUAGACCUACGU
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Wild sirna efficacy for mutant allele: | 87
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Percentage discrimination for wild siRNA: | Poor discrimination
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| Concentration: | 10mg |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P05067 |
| Target sequence: | GAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGA |
| Genbank Accesion: | NM_000484.3 |
| Cell-line: | Cos-7 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Integrated DNA Technologies |
| Post-transfection duration: | 72 hours |
| OMID ID: | 104300 |
| KEGG disease ID: | H00056 |
| Reference: | 14754988 |
