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siRNA Id: | aspsirna0197
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siRNA Name: | siA9
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| Gene: | Microtubule-associated protein tau (MAPT) |
| Disease: | Frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17) |
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Wild allele/target (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
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Mutant allele/target (5'->3'): | GUGGCCAGAUGGAAGUAAAAUC
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Target Mutation: | c.1960G>A,p.Val654Met
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Wild siRNA (As strand): | CACCGGUCCACCUUCAUUUUAG
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ASP-siRNA (As strand): | CACCGGUCUACCUUCAUUUUAG
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Mismatch position in siRNA: | U14C |
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Mutant allele (5'->3'): | GUGGCCAGAUGGAAGUAAAAUC
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ASP-siRNA (As strand): | CACCGGUCUACCUUCAUUUUAG
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ASP-siRNA efficacy for mutant allele: | 65
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Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
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ASP-siRNA (As strand): | CACCGGUCUACCUUCAUUUUAG
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ASP-siRNA efficacy for wild allele: | 60
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Percentage discrimination for ASP-siRNA: | 5
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Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
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Wild siRNA (As strand): | CACCGGUCCACCUUCAUUUUAG
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Wild sirna efficacy for wild allele: | High efficacy
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Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
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ASP-siRNA (As strand): | CACCGGUCUACCUUCAUUUUAG
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Wild sirna efficacy for mutant allele: | 87
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Percentage discrimination for wild siRNA: | Poor discrimination
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| Concentration: | 10mg |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P10636 |
| Target sequence: | ACCAGGAGGTGGCCAGGTGGAAGTAAAATCTGA |
| Genbank Accesion: | NM_016835.4 |
| Cell-line: | Cos-7 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Integrated DNA Technologies |
| Post-transfection duration: | 72 hours |
| OMID ID: | 600274 |
| KEGG disease ID: | H00078 |
| Reference: | 14754988 |
