| |
|---|
|
siRNA Id: | aspsirna0242
|
|
siRNA Name: | LRRK2 P2
|
| Gene: | Leucine rich repeat kinase 2 (LRRK2) Gene |
| Disease: | Parkinsons disease (PD) |
|
Wild allele/target (5'->3'): | CAAAGAUUGCUGACUACGG
|
|
Mutant allele/target (5'->3'): | CAAAGAUUGCUGACUACAG
|
|
Target Mutation: | c.6055G>A,p.Gly2019Ser
|
|
Wild siRNA (As strand): | GUUUCUAACGACUGAUGCC
|
|
ASP-siRNA (As strand): | GUUUCUAACGACUGAUGUC
|
|
Mismatch position in siRNA: | U2C |
|
Mutant allele (5'->3'): | CAAAGAUUGCUGACUACAG
|
|
ASP-siRNA (As strand): | GUUUCUAACGACUGAUGUC
|
|
ASP-siRNA efficacy for mutant allele: | 40
|
|
Wild allele (5'->3'): | CAAAGAUUGCUGACUACGG
|
|
ASP-siRNA (As strand): | GUUUCUAACGACUGAUGUC
|
|
ASP-siRNA efficacy for wild allele: | 20
|
|
Percentage discrimination for ASP-siRNA: | 20
|
|
Wild allele (5'->3'): | CAAAGAUUGCUGACUACGG
|
|
Wild siRNA (As strand): | GUUUCUAACGACUGAUGCC
|
|
Wild sirna efficacy for wild allele: | High efficacy
|
|
Wild allele (5'->3'): | CAAAGAUUGCUGACUACGG
|
|
ASP-siRNA (As strand): | GUUUCUAACGACUGAUGUC
|
|
Wild sirna efficacy for mutant allele: | Moderate efficacy
|
|
Percentage discrimination for wild siRNA: | Poor discrimination
|
| Concentration: | 50nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q5S007 |
| Target sequence: | UUGCAAAGAUUGCUGACUACGGCAUUGCUCAGUACUGC |
| Genbank Accesion: | NM_198578.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Sigma Genosys |
| Post-transfection duration: | 48 hours |
| OMID ID: | 168600 |
| KEGG disease ID: | H00057 |
| Reference: | 22031823 |
