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siRNA Id: | aspsirna0260
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siRNA Name: | LRRK2 P14_10C
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| Gene: | Leucine rich repeat kinase 2 (LRRK2) Gene |
| Disease: | Parkinsons disease (PD) |
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Wild allele/target (5'->3'): | ACUACGGCAUUGCUCAGUA
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Mutant allele/target (5'->3'): | ACUACAGCAGUGCUCAGUA
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Target Mutation: | c.6055G>A,p.Gly2019Ser
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Wild siRNA (As strand): | UGAUGCCGUAACGAGUCAU
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ASP-siRNA (As strand): | UGAUGUCGUCACGAGUCAU
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Mismatch position in siRNA: | U14C, C10A |
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Mutant allele (5'->3'): | ACUACAGCAGUGCUCAGUA
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ASP-siRNA (As strand): | UGAUGUCGUCACGAGUCAU
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ASP-siRNA efficacy for mutant allele: | 88
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Wild allele (5'->3'): | ACUACGGCAUUGCUCAGUA
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ASP-siRNA (As strand): | UGAUGUCGUCACGAGUCAU
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ASP-siRNA efficacy for wild allele: | 25
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Percentage discrimination for ASP-siRNA: | 63
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Wild allele (5'->3'): | ACUACGGCAUUGCUCAGUA
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Wild siRNA (As strand): | UGAUGCCGUAACGAGUCAU
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Wild sirna efficacy for wild allele: | High efficacy
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Wild allele (5'->3'): | ACUACGGCAUUGCUCAGUA
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ASP-siRNA (As strand): | UGAUGUCGUCACGAGUCAU
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Wild sirna efficacy for mutant allele: | Moderate efficacy
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Percentage discrimination for wild siRNA: | Poor discrimination
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| Concentration: | 50nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q5S007 |
| Target sequence: | UUGCAAAGAUUGCUGACUACGGCAUUGCUCAGUACUGC |
| Genbank Accesion: | NM_198578.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | Flourescence microscopy |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Sigma Genosys |
| Post-transfection duration: | 48 hours |
| OMID ID: | 168600 |
| KEGG disease ID: | H00057 |
| Reference: | 22031823 |
