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siRNA Id: | aspsirna0277
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siRNA Name: | siCOL7A1 delta 87mut4
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| Gene: | Collagen type VII alpha 1 (COL7A1) |
| Disease: | Dominant dystrophic epidermolysis bullosa(DDEB) |
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Wild allele/target (5'->3'): | GUGUGCCAGGGUCACCAGGCUGUGGUCGG
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Mutant allele/target (5'->3'): | GUGUGCCA---GCUGUGGUCGG
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Target Mutation: | c.6752G>A,p.Gly2251Glu
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Wild siRNA (As strand): | CACACGGUCCCAGUGGUCCGACACCAGCC
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ASP-siRNA (As strand): | CACACGGU---CGACACCAGCC
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Mismatch position in siRNA: | NA |
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Mutant allele (5'->3'): | GUGUGCCA---GCUGUGGUCGG
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ASP-siRNA (As strand): | CACACGGU---CGACACCAGCC
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ASP-siRNA efficacy for mutant allele: | 66
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Wild allele (5'->3'): | GUGUGCCAGGGUCACCAGGCUGUGGUCGG
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ASP-siRNA (As strand): | CACACGGU---CGACACCAGCC
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ASP-siRNA efficacy for wild allele: | 20
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Percentage discrimination for ASP-siRNA: | 46
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Wild allele (5'->3'): | GUGUGCCAGGGUCACCAGGCUGUGGUCGG
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Wild siRNA (As strand): | CACACGGUCCCAGUGGUCCGACACCAGCC
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Wild sirna efficacy for wild allele: | Moderate efficacy
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Wild allele (5'->3'): | GUGUGCCAGGGUCACCAGGCUGUGGUCGG
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ASP-siRNA (As strand): | CACACGGU---CGACACCAGCC
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Wild sirna efficacy for mutant allele: | Moderate efficacy
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Percentage discrimination for wild siRNA: | Poor discrimination
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| Concentration: | 10nM |
| Orientation: | Sense |
| Mismatch in: | nil |
| UniProt ID: | Q02388 |
| Target sequence: | GGGAGCCCUGGUGUGCCAGGGUCACCAG |
| Genbank Accesion: | NM_000094.3 |
| Cell-line: | 293T |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen,Sigma |
| Post-transfection duration: | 48 hours |
| OMID ID: | 131750 |
| KEGG disease ID: | H00584 |
| Reference: | 22336946 |
