| |
|---|
|
siRNA Id: | aspsirna0279
|
|
siRNA Name: | siCOL7A1 delta 87mut6
|
| Gene: | Collagen type VII alpha 1 (COL7A1) |
| Disease: | Dominant dystrophic epidermolysis bullosa(DDEB) |
|
Wild allele/target (5'->3'): | UGGUGUGCCAGGGUCACCAGGCUGUGGUC
|
|
Mutant allele/target (5'->3'): | UGGUGUGCCA---GCUGUGGUC
|
|
Target Mutation: | c.6752G>A,p.Gly2251Glu
|
|
Wild siRNA (As strand): | ACCACACGGUCCCAGUGGUCCGACACCAG
|
|
ASP-siRNA (As strand): | ACCACACGGU---CGACACCAG
|
|
Mismatch position in siRNA: | NA |
|
Mutant allele (5'->3'): | UGGUGUGCCA---GCUGUGGUC
|
|
ASP-siRNA (As strand): | ACCACACGGU---CGACACCAG
|
|
ASP-siRNA efficacy for mutant allele: | 60
|
|
Wild allele (5'->3'): | UGGUGUGCCAGGGUCACCAGGCUGUGGUC
|
|
ASP-siRNA (As strand): | ACCACACGGU---CGACACCAG
|
|
ASP-siRNA efficacy for wild allele: | 43
|
|
Percentage discrimination for ASP-siRNA: | 17
|
|
Wild allele (5'->3'): | UGGUGUGCCAGGGUCACCAGGCUGUGGUC
|
|
Wild siRNA (As strand): | ACCACACGGUCCCAGUGGUCCGACACCAG
|
|
Wild sirna efficacy for wild allele: | Moderate efficacy
|
|
Wild allele (5'->3'): | UGGUGUGCCAGGGUCACCAGGCUGUGGUC
|
|
ASP-siRNA (As strand): | ACCACACGGU---CGACACCAG
|
|
Wild sirna efficacy for mutant allele: | Moderate efficacy
|
|
Percentage discrimination for wild siRNA: | Poor discrimination
|
| Concentration: | 10nM |
| Orientation: | Sense |
| Mismatch in: | nil |
| UniProt ID: | Q02388 |
| Target sequence: | GGGAGCCCUGGUGUGCCAGGGUCACCAG |
| Genbank Accesion: | NM_000094.3 |
| Cell-line: | 293T |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen,Sigma |
| Post-transfection duration: | 48 hours |
| OMID ID: | 131750 |
| KEGG disease ID: | H00584 |
| Reference: | 22336946 |
