| |
|---|
|
siRNA Id: | aspsirna0322
|
|
siRNA Name: | siCOL7 G2043R mut7
|
| Gene: | Collagen type VII alpha 1 (COL7A1) |
| Disease: | Dominant dystrophic epidermolysis bullosa(DDEB) |
|
Wild allele/target (5'->3'): | CCUGGUAUUCCCGGGCUCC
|
|
Mutant allele/target (5'->3'): | CCUGGUAUUCCCAGGCUCC
|
|
Target Mutation: | c.6127G>A,p.Gly2043Arg
|
|
Wild siRNA (As strand): | GGACCAUAAGGGCCCGAGG
|
|
ASP-siRNA (As strand): | GGACCAUAAGGGUCCGAGG
|
|
Mismatch position in siRNA: | U13C |
|
Mutant allele (5'->3'): | CCUGGUAUUCCCAGGCUCC
|
|
ASP-siRNA (As strand): | GGACCAUAAGGGUCCGAGG
|
|
ASP-siRNA efficacy for mutant allele: | 50
|
|
Wild allele (5'->3'): | CCUGGUAUUCCCGGGCUCC
|
|
ASP-siRNA (As strand): | GGACCAUAAGGGUCCGAGG
|
|
ASP-siRNA efficacy for wild allele: | 20
|
|
Percentage discrimination for ASP-siRNA: | 30
|
|
Wild allele (5'->3'): | CCUGGUAUUCCCGGGCUCC
|
|
Wild siRNA (As strand): | GGACCAUAAGGGCCCGAGG
|
|
Wild sirna efficacy for wild allele: | 70
|
|
Wild allele (5'->3'): | CCUGGUAUUCCCGGGCUCC
|
|
ASP-siRNA (As strand): | GGACCAUAAGGGUCCGAGG
|
|
Wild sirna efficacy for mutant allele: | 88
|
|
Percentage discrimination for wild siRNA: | -18
|
| Concentration: | 10nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q02388 |
| Target sequence: | GGAAAGCCUGGUAUUCCCGGGCUCCCAGGCAGGGCUG |
| Genbank Accesion: | NM_000094.3 |
| Cell-line: | 293T |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen,Sigma |
| Post-transfection duration: | 48 hours |
| OMID ID: | 131750 |
| KEGG disease ID: | H00584 |
| Reference: | 22336946 |
