aspsirna0330 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0330
siRNA Name: siCOL7 G2043R mut15
Gene:Collagen type VII alpha 1 (COL7A1)
Disease:Dominant dystrophic epidermolysis bullosa(DDEB)
Wild allele/target (5'->3'): UCCCGGGCUCCCAGGCAGG
Mutant allele/target (5'->3'):UCCCAGGCUCCCAGGCAGG
Target Mutation: c.6127G>A,p.Gly2043Arg
Wild siRNA (As strand):AGGGCCCGAGGGUCCGUCC
ASP-siRNA (As strand):AGGGUCCGAGGGUCCGUCC
Mismatch position in siRNA: U5C
Mutant allele (5'->3'): UCCCAGGCUCCCAGGCAGG
ASP-siRNA (As strand):AGGGUCCGAGGGUCCGUCC
ASP-siRNA efficacy for mutant allele:24
Wild allele (5'->3'): UCCCGGGCUCCCAGGCAGG
ASP-siRNA (As strand):AGGGUCCGAGGGUCCGUCC
ASP-siRNA efficacy for wild allele:10
Percentage discrimination for ASP-siRNA:14
Wild allele (5'->3'): UCCCGGGCUCCCAGGCAGG
Wild siRNA (As strand):AGGGCCCGAGGGUCCGUCC
Wild sirna efficacy for wild allele:70
Wild allele (5'->3'): UCCCGGGCUCCCAGGCAGG
ASP-siRNA (As strand):AGGGUCCGAGGGUCCGUCC
Wild sirna efficacy for mutant allele:88
Percentage discrimination for wild siRNA:-18
Concentration:10nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: Q02388
Target sequence: GGAAAGCCUGGUAUUCCCGGGCUCCCAGGCAGGGCUG
Genbank Accesion: NM_000094.3
Cell-line: 293T
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen,Sigma
Post-transfection duration: 48 hours
OMID ID: 131750
KEGG disease ID:H00584
Reference:22336946

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