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siRNA Id: | aspsirna0340
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siRNA Name: | SiRNA-mut
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| Gene: | Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE) |
| Disease: | Sialuria |
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Wild allele/target (5'->3'): | GAGUGAUGCGGAAGAAGGG
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Mutant allele/target (5'->3'): | GAGUGAUGCAGAAGAAGGG
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Target Mutation: | c.830G>A,p.Arg277Gln
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Wild siRNA (As strand): | CUCACUACGCCUUCUUCCC
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ASP-siRNA (As strand): | CUCACUACGUCUUCUUCCC
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Mismatch position in siRNA: | U10C |
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Mutant allele (5'->3'): | GAGUGAUGCAGAAGAAGGG
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ASP-siRNA (As strand): | CUCACUACGUCUUCUUCCC
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ASP-siRNA efficacy for mutant allele: | 85
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Wild allele (5'->3'): | GAGUGAUGCGGAAGAAGGG
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ASP-siRNA (As strand): | CUCACUACGUCUUCUUCCC
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ASP-siRNA efficacy for wild allele: | 10
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Percentage discrimination for ASP-siRNA: | 75
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Wild allele (5'->3'): | GAGUGAUGCGGAAGAAGGG
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Wild siRNA (As strand): | CUCACUACGCCUUCUUCCC
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Wild sirna efficacy for wild allele: | 33
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Wild allele (5'->3'): | GAGUGAUGCGGAAGAAGGG
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ASP-siRNA (As strand): | CUCACUACGUCUUCUUCCC
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Wild sirna efficacy for mutant allele: | 18
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Percentage discrimination for wild siRNA: | 15
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| Concentration: | 1.5mg |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q9Y223 |
| Target sequence: | GATGGTTCGAGUGAUGCGGAAGAAGGG |
| Genbank Accesion: | NM_001128227.2 |
| Cell-line: | Fibroblast |
| Experimental technique used: | GNE enzyme assay |
| Transfection method: | Nucleofector solution |
| siRNA expression method: | Qiagen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 269921 |
| KEGG disease ID: | H00147 |
| Reference: | 18653764 |
