| |
|---|
|
siRNA Id: | aspsirna0346
|
|
siRNA Name: | SEQUENCE4
|
| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
|
Wild allele/target (5'->3'): | GAGGAAGAGGAGGAGGAGGCCGAC
|
|
Mutant allele/target (5'->3'): | GAGGAAGAGGAGGAG---GCCGAC
|
|
Target Mutation: | c.52CAG(36_39)
|
|
Wild siRNA (As strand): | CUCCUUCUCCUCCUCCUCCGGCUG
|
|
ASP-siRNA (As strand): | CUCCUUCUCCUCCUC---CGGCUG
|
|
Mismatch position in siRNA: | NA |
|
Mutant allele (5'->3'): | GAGGAAGAGGAGGAG---GCCGAC
|
|
ASP-siRNA (As strand): | CUCCUUCUCCUCCUC---CGGCUG
|
|
ASP-siRNA efficacy for mutant allele: | 74
|
|
Wild allele (5'->3'): | GAGGAAGAGGAGGAGGAGGCCGAC
|
|
ASP-siRNA (As strand): | CUCCUUCUCCUCCUC---CGGCUG
|
|
ASP-siRNA efficacy for wild allele: | 72
|
|
Percentage discrimination for ASP-siRNA: | 2
|
|
Wild allele (5'->3'): | GAGGAAGAGGAGGAGGAGGCCGAC
|
|
Wild siRNA (As strand): | CUCCUUCUCCUCCUCCUCCGGCUG
|
|
Wild sirna efficacy for wild allele: | 33
|
|
Wild allele (5'->3'): | GAGGAAGAGGAGGAGGAGGCCGAC
|
|
ASP-siRNA (As strand): | CUCCUUCUCCUCCUC---CGGCUG
|
|
Wild sirna efficacy for mutant allele: | 18
|
|
Percentage discrimination for wild siRNA: | 15
|
| Concentration: | 20nM |
| Orientation: | Sense |
| Mismatch in: | nil |
| UniProt ID: | Q96CV9 |
| Target sequence: | GACGAGGAAGAGGAGGAGGAGGCCGACGCCC |
| Genbank Accesion: | NM_002111.6 |
| Cell-line: | Fibroblast |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 40 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H01243 |
| Reference: | 19094060 |
