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siRNA Id: | aspsirna0349
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siRNA Name: | Si2
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| Gene: | Collagen, type III, alpha 1 (COL3A1) |
| Disease: | Vascular Ehlers-Danlos Syndrome (vEDs) |
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Wild allele/target (5'->3'): | GGUCCAGCUGGGAUACCUG
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Mutant allele/target (5'->3'): | GUUCCAGCUGGGAUACCUG
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Target Mutation: | c.764G>T,p.Gly255Val
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Wild siRNA (As strand): | CCAGGUCGACCCUAUGGAC
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ASP-siRNA (As strand): | CAAGGUCGACCCUAUGGAC
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Mismatch position in siRNA: | A18C |
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Mutant allele (5'->3'): | GUUCCAGCUGGGAUACCUG
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ASP-siRNA (As strand): | CAAGGUCGACCCUAUGGAC
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ASP-siRNA efficacy for mutant allele: | 50
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Wild allele (5'->3'): | GGUCCAGCUGGGAUACCUG
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ASP-siRNA (As strand): | CAAGGUCGACCCUAUGGAC
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ASP-siRNA efficacy for wild allele: | 58
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Percentage discrimination for ASP-siRNA: | -8
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Wild allele (5'->3'): | GGUCCAGCUGGGAUACCUG
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Wild siRNA (As strand): | CCAGGUCGACCCUAUGGAC
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Wild sirna efficacy for wild allele: | 47
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Wild allele (5'->3'): | GGUCCAGCUGGGAUACCUG
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ASP-siRNA (As strand): | CAAGGUCGACCCUAUGGAC
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Wild sirna efficacy for mutant allele: | 20
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Percentage discrimination for wild siRNA: | 27
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| Concentration: | 100nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P02461 |
| Target sequence: | CCUGGACCUCCAGGUAUCAAAGGUCCAGCUGGGAUACCUGGAU |
| Genbank Accesion: | NM_000090.3 |
| Cell-line: | Fibroblast |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Dharmafect 1 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 130050 |
| KEGG disease ID: | H00802 |
| Reference: | 22038052 |
