| |
|---|
|
siRNA Id: | aspsirna0351
|
|
siRNA Name: | Si4
|
| Gene: | Collagen, type III, alpha 1 (COL3A1) |
| Disease: | Vascular Ehlers-Danlos Syndrome (vEDs) |
|
Wild allele/target (5'->3'): | AAGGUCCAGCUGGGAUACC
|
|
Mutant allele/target (5'->3'): | AAGUUCCAGCUGGGAUACC
|
|
Target Mutation: | c.764G>T,p.Gly255Val
|
|
Wild siRNA (As strand): | UUCCAGGUCGACCCUAUGG
|
|
ASP-siRNA (As strand): | UUCAAGGUCGACCCUAUGG
|
|
Mismatch position in siRNA: | A16C |
|
Mutant allele (5'->3'): | AAGUUCCAGCUGGGAUACC
|
|
ASP-siRNA (As strand): | UUCAAGGUCGACCCUAUGG
|
|
ASP-siRNA efficacy for mutant allele: | 45
|
|
Wild allele (5'->3'): | AAGGUCCAGCUGGGAUACC
|
|
ASP-siRNA (As strand): | UUCAAGGUCGACCCUAUGG
|
|
ASP-siRNA efficacy for wild allele: | 0
|
|
Percentage discrimination for ASP-siRNA: | 45
|
|
Wild allele (5'->3'): | AAGGUCCAGCUGGGAUACC
|
|
Wild siRNA (As strand): | UUCCAGGUCGACCCUAUGG
|
|
Wild sirna efficacy for wild allele: | 47
|
|
Wild allele (5'->3'): | AAGGUCCAGCUGGGAUACC
|
|
ASP-siRNA (As strand): | UUCAAGGUCGACCCUAUGG
|
|
Wild sirna efficacy for mutant allele: | 20
|
|
Percentage discrimination for wild siRNA: | 27
|
| Concentration: | 100nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P02461 |
| Target sequence: | CCUGGACCUCCAGGUAUCAAAGGUCCAGCUGGGAUACCUGGAU |
| Genbank Accesion: | NM_000090.3 |
| Cell-line: | Fibroblast |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Dharmafect 1 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 130050 |
| KEGG disease ID: | H00802 |
| Reference: | 22038052 |
