| |
|---|
|
siRNA Id: | aspsirna0376
|
|
siRNA Name: | siR206H_A10
|
| Gene: | Activin A receptor type I (ACVR1) |
| Disease: | Fibrodysplasia Ossificans Progressiva (FOP) |
|
Wild allele/target (5'->3'): | CAGUGGCUCGCCAGAUUAC
|
|
Mutant allele/target (5'->3'): | CAGUGGCUCACCAGAUUAC
|
|
Target Mutation: | c.617G>A,p.Arg206His
|
|
Wild siRNA (As strand): | GUCACCGAGCGGUCUAAUG
|
|
ASP-siRNA (As strand): | GUCACCGAGUGGUCUAAUG
|
|
Mismatch position in siRNA: | U10C |
|
Mutant allele (5'->3'): | CAGUGGCUCACCAGAUUAC
|
|
ASP-siRNA (As strand): | GUCACCGAGUGGUCUAAUG
|
|
ASP-siRNA efficacy for mutant allele: | NA
|
|
Wild allele (5'->3'): | CAGUGGCUCGCCAGAUUAC
|
|
ASP-siRNA (As strand): | GUCACCGAGUGGUCUAAUG
|
|
ASP-siRNA efficacy for wild allele: | NA
|
|
Percentage discrimination for ASP-siRNA: | NA
|
|
Wild allele (5'->3'): | CAGUGGCUCGCCAGAUUAC
|
|
Wild siRNA (As strand): | GUCACCGAGCGGUCUAAUG
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | CAGUGGCUCGCCAGAUUAC
|
|
ASP-siRNA (As strand): | GUCACCGAGUGGUCUAAUG
|
|
Wild sirna efficacy for mutant allele: | NA
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 20nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | Q06900 |
| Target sequence: | AAGAACAGTGGCTCGCCAGATTACACTGT |
| Genbank Accesion: | NM_001105.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Sigma-aldrich |
| Post-transfection duration: | 24 hours |
| OMID ID: | 135100 |
| KEGG disease ID: | H00430 |
| Reference: | 22130450 |
