| |
|---|
|
siRNA Id: | aspsirna0390
|
|
siRNA Name: | siG356D_A10 (G16)
|
| Gene: | Activin A receptor type I (ACVR1) |
| Disease: | Fibrodysplasia Ossificans Progressiva (FOP) |
|
Wild allele/target (5'->3'): | CAGAUUUGGGCCUGGCAGU
|
|
Mutant allele/target (5'->3'): | CAGAUUUGGACCUGGGAGU
|
|
Target Mutation: | c.1067G>A,p.Gly356Asp
|
|
Wild siRNA (As strand): | GUCUAAACCCGGACCGUCA
|
|
ASP-siRNA (As strand): | GUCUAAACCUGGACCCUCA
|
|
Mismatch position in siRNA: | C4G,U10C |
|
Mutant allele (5'->3'): | CAGAUUUGGACCUGGGAGU
|
|
ASP-siRNA (As strand): | GUCUAAACCUGGACCCUCA
|
|
ASP-siRNA efficacy for mutant allele: | 92
|
|
Wild allele (5'->3'): | CAGAUUUGGGCCUGGCAGU
|
|
ASP-siRNA (As strand): | GUCUAAACCUGGACCCUCA
|
|
ASP-siRNA efficacy for wild allele: | 30
|
|
Percentage discrimination for ASP-siRNA: | 62
|
|
Wild allele (5'->3'): | CAGAUUUGGGCCUGGCAGU
|
|
Wild siRNA (As strand): | GUCUAAACCCGGACCGUCA
|
|
Wild sirna efficacy for wild allele: | 69
|
|
Wild allele (5'->3'): | CAGAUUUGGGCCUGGCAGU
|
|
ASP-siRNA (As strand): | GUCUAAACCUGGACCCUCA
|
|
Wild sirna efficacy for mutant allele: | 38
|
|
Percentage discrimination for wild siRNA: | 31
|
| Concentration: | 20nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | Q06900 |
| Target sequence: | GCATAGCAGATTTGGGCCTGGCAGTCATG |
| Genbank Accesion: | NM_001105.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Sigma-aldrich |
| Post-transfection duration: | 24 hours |
| OMID ID: | 135100 |
| KEGG disease ID: | H00430 |
| Reference: | 22130450 |
