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siRNA Id: | aspsirna0426
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siRNA Name: | siG356D_A9_5+
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| Gene: | Activin A receptor type I (ACVR1) |
| Disease: | Fibrodysplasia Ossificans Progressiva (FOP) |
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Wild allele/target (5'->3'): | AGAUUUGGGCCUGGCAGUC
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Mutant allele/target (5'->3'): | CAGAUUUGGACCUGGCAGUC
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Target Mutation: | c.1067G>A,p.Gly356Asp
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Wild siRNA (As strand): | UCUAAACCCGGACCGUCAG
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ASP-siRNA (As strand): | UCUAAACCUGGACCGUCAG
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Mismatch position in siRNA: | U11C |
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Mutant allele (5'->3'): | CAGAUUUGGACCUGGCAGUC
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ASP-siRNA (As strand): | UCUAAACCUGGACCGUCAG
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ASP-siRNA efficacy for mutant allele: | 85
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Wild allele (5'->3'): | AGAUUUGGGCCUGGCAGUC
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ASP-siRNA (As strand): | UCUAAACCUGGACCGUCAG
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ASP-siRNA efficacy for wild allele: | 48
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Percentage discrimination for ASP-siRNA: | 37
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Wild allele (5'->3'): | AGAUUUGGGCCUGGCAGUC
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Wild siRNA (As strand): | UCUAAACCCGGACCGUCAG
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Wild sirna efficacy for wild allele: | 69
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Wild allele (5'->3'): | AGAUUUGGGCCUGGCAGUC
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ASP-siRNA (As strand): | UCUAAACCUGGACCGUCAG
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Wild sirna efficacy for mutant allele: | 38
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Percentage discrimination for wild siRNA: | 31
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| Concentration: | 20nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | Q06900 |
| Target sequence: | GCATAGCAGATTTGGGCCTGGCAGTCATG |
| Genbank Accesion: | NM_001105.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Sigma-aldrich |
| Post-transfection duration: | 24 hours |
| OMID ID: | 135100 |
| KEGG disease ID: | H00430 |
| Reference: | 22130450 |
