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siRNA Id: | aspsirna0431
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siRNA Name: | P4
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| Gene: | Presenilin1 (PSEN1) |
| Disease: | Alzheimers disease |
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Wild allele/target (5'->3'): | GUUCUGGUUGGUAAAGCCU
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Mutant allele/target (5'->3'): | GUUGUGGUUGGUAAAGCCU
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Target Mutation: | c.811C>G, p.Leu271Val
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Wild siRNA (As strand): | CAAGACCAACCAUUUCGGA
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ASP-siRNA (As strand): | CAACACCAACCAUUUCGGA
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Mismatch position in siRNA: | C16G |
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Mutant allele (5'->3'): | GUUGUGGUUGGUAAAGCCU
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ASP-siRNA (As strand): | CAACACCAACCAUUUCGGA
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ASP-siRNA efficacy for mutant allele: | 72
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Wild allele (5'->3'): | GUUCUGGUUGGUAAAGCCU
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ASP-siRNA (As strand): | CAACACCAACCAUUUCGGA
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ASP-siRNA efficacy for wild allele: | 28
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Percentage discrimination for ASP-siRNA: | 44
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Wild allele (5'->3'): | GUUCUGGUUGGUAAAGCCU
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Wild siRNA (As strand): | CAAGACCAACCAUUUCGGA
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | GUUCUGGUUGGUAAAGCCU
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ASP-siRNA (As strand): | CAACACCAACCAUUUCGGA
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 1.5microL |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P49768 |
| Target sequence: | UUCAUUUUCUACAGUGUUCUGGUUGGUAAAGCCUCAG |
| Genbank Accesion: | NM_000021.3 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized (Phosphoramidite method) |
| Post-transfection duration: | 48 hours |
| OMID ID: | 104300 |
| KEGG disease ID: | H00056 |
| Reference: | 21559198 |
