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siRNA Id: | aspsirna0464
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siRNA Name: | K5_541.c18
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| Gene: | Keratin 5 (KRT5) |
| Disease: | Epidermolysis bullosa simplex (EBS) |
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Wild allele/target (5'->3'): | CUCCUUCAUCGACAAGGUG
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Mutant allele/target (5'->3'): | CCCCUUCAUCGACAAGGUG
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Target Mutation: | c.541T>C ,p.Ser181Pro
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Wild siRNA (As strand): | GAGGAAGUAGCUGUUCCAC
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ASP-siRNA (As strand): | GGGGAAGUAGCUGUUCCAC
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Mismatch position in siRNA: | G18A |
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Mutant allele (5'->3'): | CCCCUUCAUCGACAAGGUG
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ASP-siRNA (As strand): | GGGGAAGUAGCUGUUCCAC
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ASP-siRNA efficacy for mutant allele: | 62
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Wild allele (5'->3'): | CUCCUUCAUCGACAAGGUG
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ASP-siRNA (As strand): | GGGGAAGUAGCUGUUCCAC
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ASP-siRNA efficacy for wild allele: | 35
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Percentage discrimination for ASP-siRNA: | 27
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Wild allele (5'->3'): | CUCCUUCAUCGACAAGGUG
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Wild siRNA (As strand): | GAGGAAGUAGCUGUUCCAC
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CUCCUUCAUCGACAAGGUG
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ASP-siRNA (As strand): | GGGGAAGUAGCUGUUCCAC
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 10 pM -6.25microM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P13647 |
| Target sequence: | GACCCUCAACAAUAAGUUUGCCUCCUUCAUCGACAAGGUGCGG |
| Genbank Accesion: | NM_000424.3 |
| Cell-line: | AD293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Eurofins MWG Operon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 131760 |
| KEGG disease ID: | H00584 |
| Reference: | 21716320 |
