| |
|---|
|
siRNA Id: | aspsirna0509
|
|
siRNA Name: | K5_579.g6
|
| Gene: | Keratin 5 (KRT5) |
| Disease: | Epidermolysis bullosa simplex (EBS) |
|
Wild allele/target (5'->3'): | UGGAGCAGCAGAACAAGGU
|
|
Mutant allele/target (5'->3'): | UGGAGCAGCAGAAGAAGGU
|
|
Target Mutation: | c.579C>G, p.Asn193Lys
|
|
Wild siRNA (As strand): | ACCUCGUCGUCUUGUUCCA
|
|
ASP-siRNA (As strand): | ACCUCGUCGUCUUCUUCCA
|
|
Mismatch position in siRNA: | C6G |
|
Mutant allele (5'->3'): | UGGAGCAGCAGAAGAAGGU
|
|
ASP-siRNA (As strand): | ACCUCGUCGUCUUCUUCCA
|
|
ASP-siRNA efficacy for mutant allele: | 50
|
|
Wild allele (5'->3'): | UGGAGCAGCAGAACAAGGU
|
|
ASP-siRNA (As strand): | ACCUCGUCGUCUUCUUCCA
|
|
ASP-siRNA efficacy for wild allele: | 0
|
|
Percentage discrimination for ASP-siRNA: | 50
|
|
Wild allele (5'->3'): | UGGAGCAGCAGAACAAGGU
|
|
Wild siRNA (As strand): | ACCUCGUCGUCUUGUUCCA
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | UGGAGCAGCAGAACAAGGU
|
|
ASP-siRNA (As strand): | ACCUCGUCGUCUUCUUCCA
|
|
Wild sirna efficacy for mutant allele: | NA
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 10 pM -6.25microM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P13647 |
| Target sequence: | GUGCGGUUCCUGGAGCAGCAGAACAAGGUUCUGGACACCAAGU |
| Genbank Accesion: | NM_000424.3 |
| Cell-line: | HaCaT |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Eurofins MWG Operon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 131760 |
| KEGG disease ID: | H00584 |
| Reference: | 21716320 |
