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siRNA Id: | aspsirna0528
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siRNA Name: | Si9,10
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| Gene: | Synuclein, alpha (SNCA) |
| Disease: | Parkinsons disease (PD) |
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Wild allele/target (5'->3'): | AUGGUGUGGCAACAGUGGCUG
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Mutant allele/target (5'->3'): | AUGGUGUGAGAACAGUGGCUG
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Target Mutation: | c.157G>A,p.Ala53Thr
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Wild siRNA (As strand): | UACCACACCGUUGUCACCGAC
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ASP-siRNA (As strand): | UACCACACUCUUGUCACCGAC
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Mismatch position in siRNA: | U13C,C12G |
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Mutant allele (5'->3'): | AUGGUGUGAGAACAGUGGCUG
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ASP-siRNA (As strand): | UACCACACUCUUGUCACCGAC
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ASP-siRNA efficacy for mutant allele: | 75
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Wild allele (5'->3'): | AUGGUGUGGCAACAGUGGCUG
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ASP-siRNA (As strand): | UACCACACUCUUGUCACCGAC
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ASP-siRNA efficacy for wild allele: | 60
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Percentage discrimination for ASP-siRNA: | 15
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Wild allele (5'->3'): | AUGGUGUGGCAACAGUGGCUG
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Wild siRNA (As strand): | UACCACACCGUUGUCACCGAC
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | AUGGUGUGGCAACAGUGGCUG
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ASP-siRNA (As strand): | UACCACACUCUUGUCACCGAC
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 0.1nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P37840 |
| Target sequence: | GTGGTGCAUGGUGUGGCAACAGUGGCUG |
| Genbank Accesion: | NM_000345.3 |
| Cell-line: | HeLa |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | QiagenXeragon |
| Post-transfection duration: | 48 hours |
| OMID ID: | 168600 |
| KEGG disease ID: | H00057 |
| Reference: | 16455076 |
