aspsirna0538 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0538
siRNA Name: Si9,12
Gene:Synuclein, alpha (SNCA)
Disease:Parkinsons disease (PD)
Wild allele/target (5'->3'): AUGGUGUGGCAACAGUGGCUG
Mutant allele/target (5'->3'):AUGGUGUGACAUCAGUGGCUG
Target Mutation: c.157G>A,p.Ala53Thr
Wild siRNA (As strand):UACCACACCGUUGUCACCGAC
ASP-siRNA (As strand):UACCACACUGUAGUCACCGAC
Mismatch position in siRNA: U13C,A10U
Mutant allele (5'->3'): AUGGUGUGACAUCAGUGGCUG
ASP-siRNA (As strand):UACCACACUGUAGUCACCGAC
ASP-siRNA efficacy for mutant allele:51
Wild allele (5'->3'): AUGGUGUGGCAACAGUGGCUG
ASP-siRNA (As strand):UACCACACUGUAGUCACCGAC
ASP-siRNA efficacy for wild allele:2
Percentage discrimination for ASP-siRNA:49
Wild allele (5'->3'): AUGGUGUGGCAACAGUGGCUG
Wild siRNA (As strand):UACCACACCGUUGUCACCGAC
Wild sirna efficacy for wild allele:NA
Wild allele (5'->3'): AUGGUGUGGCAACAGUGGCUG
ASP-siRNA (As strand):UACCACACUGUAGUCACCGAC
Wild sirna efficacy for mutant allele:NA
Percentage discrimination for wild siRNA:NA
Concentration:0.1nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: P37840
Target sequence: GTGGTGCAUGGUGUGGCAACAGUGGCUG
Genbank Accesion: NM_000345.3
Cell-line: SH-SY5Y
Experimental technique used: Western blot
Transfection method: Lipofectamine 2000
siRNA expression method: Qiagen–Xeragon
Post-transfection duration: 72 hours
OMID ID: 168600
KEGG disease ID:H00057
Reference:16455076

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