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siRNA Id: | aspsirna0543
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siRNA Name: | SM2-RC(11)
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| Gene: | Leucine rich repeat kinase 2 (LRRK2) |
| Disease: | Parkinsons disease (PD) |
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Wild allele/target (5'->3'): | UAAAGGCUCGCGCUUCUUC
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Mutant allele/target (5'->3'): | UAAAGGCUUGCGCUUCUUC
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Target Mutation: | c.4321C>T,p.Arg1441Cys
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Wild siRNA (As strand): | AUUUCCGAGCGCGAAGAAG
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ASP-siRNA (As strand): | AUUUCCGAACGCGAAGAAG
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Mismatch position in siRNA: | A11G |
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Mutant allele (5'->3'): | UAAAGGCUUGCGCUUCUUC
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ASP-siRNA (As strand): | AUUUCCGAACGCGAAGAAG
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ASP-siRNA efficacy for mutant allele: | 63
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Wild allele (5'->3'): | UAAAGGCUCGCGCUUCUUC
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ASP-siRNA (As strand): | AUUUCCGAACGCGAAGAAG
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ASP-siRNA efficacy for wild allele: | 75
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Percentage discrimination for ASP-siRNA: | -12
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Wild allele (5'->3'): | UAAAGGCUCGCGCUUCUUC
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Wild siRNA (As strand): | AUUUCCGAGCGCGAAGAAG
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | UAAAGGCUCGCGCUUCUUC
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ASP-siRNA (As strand): | AUUUCCGAACGCGAAGAAG
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 400ng |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q5S007 |
| Target sequence: | CCTTGGCTCTTCAATATAAAGGCTCGCGCTTCTTCTTCCCCTG |
| Genbank Accesion: | NM_198578.3 |
| Cell-line: | 293T |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Arrest-In reagent |
| siRNA expression method: | Open-Biosystems |
| Post-transfection duration: | 48 hours |
| OMID ID: | 168600 |
| KEGG disease ID: | H00057 |
| Reference: | 21712955 |
