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siRNA Id: | aspsirna0546
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siRNA Name: | SM2-GS(16)
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Gene: | Leucine rich repeat kinase 2 (LRRK2) |
Disease: | Parkinsons disease (PD) |
Wild allele/target (5'->3'): | UACGGCAUUGCUCAGUACU
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Mutant allele/target (5'->3'): | UACAGCAUUGCUCAGUACU
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Target Mutation: | c.6055G>A,p.Gly2019Ser
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Wild siRNA (As strand): | AUGCCGUAACGAGUCAUGA
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ASP-siRNA (As strand): | AUGUCGUAACGAGUCAUGA
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Mismatch position in siRNA: | U16C |
Mutant allele (5'->3'): | UACAGCAUUGCUCAGUACU
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ASP-siRNA (As strand): | AUGUCGUAACGAGUCAUGA
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ASP-siRNA efficacy for mutant allele: | 46
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Wild allele (5'->3'): | UACGGCAUUGCUCAGUACU
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ASP-siRNA (As strand): | AUGUCGUAACGAGUCAUGA
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ASP-siRNA efficacy for wild allele: | 14
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Percentage discrimination for ASP-siRNA: | 32
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Wild allele (5'->3'): | UACGGCAUUGCUCAGUACU
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Wild siRNA (As strand): | AUGCCGUAACGAGUCAUGA
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | UACGGCAUUGCUCAGUACU
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ASP-siRNA (As strand): | AUGUCGUAACGAGUCAUGA
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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Concentration: | 400ng |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | Q5S007 |
Target sequence: | ATCATCGCAAAGATTGCTGACTACGGCATTGCTCAGTAC |
Genbank Accesion: | NM_198578.3 |
Cell-line: | 293T |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Arrest-In reagent |
siRNA expression method: | Open-Biosystems |
Post-transfection duration: | 48 hours |
OMID ID: | 168600 |
KEGG disease ID: | H00057 |
Reference: | 21712955 |