aspsirna0546 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0546
siRNA Name: SM2-GS(16)
Gene:Leucine rich repeat kinase 2 (LRRK2)
Disease:Parkinsons disease (PD)
Wild allele/target (5'->3'): UACGGCAUUGCUCAGUACU
Mutant allele/target (5'->3'):UACAGCAUUGCUCAGUACU
Target Mutation: c.6055G>A,p.Gly2019Ser
Wild siRNA (As strand):AUGCCGUAACGAGUCAUGA
ASP-siRNA (As strand):AUGUCGUAACGAGUCAUGA
Mismatch position in siRNA: U16C
Mutant allele (5'->3'): UACAGCAUUGCUCAGUACU
ASP-siRNA (As strand):AUGUCGUAACGAGUCAUGA
ASP-siRNA efficacy for mutant allele:46
Wild allele (5'->3'): UACGGCAUUGCUCAGUACU
ASP-siRNA (As strand):AUGUCGUAACGAGUCAUGA
ASP-siRNA efficacy for wild allele:14
Percentage discrimination for ASP-siRNA:32
Wild allele (5'->3'): UACGGCAUUGCUCAGUACU
Wild siRNA (As strand):AUGCCGUAACGAGUCAUGA
Wild sirna efficacy for wild allele:NA
Wild allele (5'->3'): UACGGCAUUGCUCAGUACU
ASP-siRNA (As strand):AUGUCGUAACGAGUCAUGA
Wild sirna efficacy for mutant allele:NA
Percentage discrimination for wild siRNA:NA
Concentration:400ng
Orientation:Sense
Mismatch in:siRNA
UniProt ID: Q5S007
Target sequence: ATCATCGCAAAGATTGCTGACTACGGCATTGCTCAGTAC
Genbank Accesion: NM_198578.3
Cell-line: 293T
Experimental technique used: Dual luciferase reporter assay
Transfection method: Arrest-In reagent
siRNA expression method: Open-Biosystems
Post-transfection duration: 48 hours
OMID ID: 168600
KEGG disease ID:H00057
Reference:21712955

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