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siRNA Id: | aspsirna0549
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siRNA Name: | siRNA1
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| Gene: | Collagen type I alpha 2 (COL1A2) |
| Disease: | Osteogenesis Imperfecta (OI) |
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Wild allele/target (5'->3'): | UGGAAAAGGAGUUGGACUU
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Mutant allele/target (5'->3'): | CGGAAAAGGAGUUGGACUU
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Target Mutation: | c.246T>C,NP_000080.2:p.Asp82=
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Wild siRNA (As strand): | ACCUUUUCCUCAACCUGAA
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ASP-siRNA (As strand): | GCCUUUUCCUCAACCUGAA
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Mismatch position in siRNA: | G19A |
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Mutant allele (5'->3'): | CGGAAAAGGAGUUGGACUU
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ASP-siRNA (As strand): | GCCUUUUCCUCAACCUGAA
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ASP-siRNA efficacy for mutant allele: | 87
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Wild allele (5'->3'): | UGGAAAAGGAGUUGGACUU
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ASP-siRNA (As strand): | GCCUUUUCCUCAACCUGAA
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ASP-siRNA efficacy for wild allele: | NA
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Percentage discrimination for ASP-siRNA: | NA
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Wild allele (5'->3'): | UGGAAAAGGAGUUGGACUU
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Wild siRNA (As strand): | ACCUUUUCCUCAACCUGAA
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | UGGAAAAGGAGUUGGACUU
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ASP-siRNA (As strand): | GCCUUUUCCUCAACCUGAA
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 0.6microg |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P08123 |
| Target sequence: | TGGGAACTTTGCTGCTCAGTATGATGGAAAAGGAGTTGGACTT |
| Genbank Accesion: | NM_000089.3 |
| Cell-line: | Primary human bone |
| Experimental technique used: | RT-PCR |
| Transfection method: | Magnet asisted transfection (MATRA) |
| siRNA expression method: | Ambion |
| Post-transfection duration: | 48 hours |
| OMID ID: | 166210 |
| KEGG disease ID: | H00506 |
| Reference: | 19015742 |
