aspsirna0556 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0556
siRNA Name: MutA-si
Gene:Torsin family 1, member A (TOR1A)
Disease:DYT1 dystonia
Wild allele/target (5'->3'): CAGAGUGGCUGAGGAGAUGAC
Mutant allele/target (5'->3'):CAGAGUGGCU---GAGAUGAC
Target Mutation: c.907_909delGAG,p.Glu303del
Wild siRNA (As strand):GUCUCACCGACUCCUCUACUG
ASP-siRNA (As strand):GUCUCACCGA---CUCUACUG
Mismatch position in siRNA: DeltaGAG
Mutant allele (5'->3'): CAGAGUGGCU---GAGAUGAC
ASP-siRNA (As strand):GUCUCACCGA---CUCUACUG
ASP-siRNA efficacy for mutant allele:80
Wild allele (5'->3'): CAGAGUGGCUGAGGAGAUGAC
ASP-siRNA (As strand):GUCUCACCGA---CUCUACUG
ASP-siRNA efficacy for wild allele:45
Percentage discrimination for ASP-siRNA:35
Wild allele (5'->3'): CAGAGUGGCUGAGGAGAUGAC
Wild siRNA (As strand):GUCUCACCGACUCCUCUACUG
Wild sirna efficacy for wild allele:0
Wild allele (5'->3'): CAGAGUGGCUGAGGAGAUGAC
ASP-siRNA (As strand):GUCUCACCGA---CUCUACUG
Wild sirna efficacy for mutant allele:82
Percentage discrimination for wild siRNA:-82
Concentration:5microg
Orientation:Sense
Mismatch in:siRNA
UniProt ID: O14656
Target sequence: GUAAGCAGAGUGGCUGAGGAGAUGACAUUUUUCCCCAAAGAG
Genbank Accesion: NM_000113.2
Cell-line: Cos-7
Experimental technique used: Western blot
Transfection method: Lipofectamine Plus
siRNA expression method: Epicentre Technologies
Post-transfection duration: 36-48 hours
OMID ID: 128100
KEGG disease ID:H01255
Reference:12783425

.
.
.
.
.
.
.
.
.
.
.

.

.

.