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siRNA Id: | aspsirna0558
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siRNA Name: | MutC-si
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| Gene: | Torsin family 1, member A (TOR1A) |
| Disease: | DYT1 dystonia |
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Wild allele/target (5'->3'): | GAGUGGCUGAGGAGAUGACAUUUUUC
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Mutant allele/target (5'->3'): | GAGUGGCU---GAGAUGACAUUUUUC
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Target Mutation: | c.907_909delGAG,p.Glu303del
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Wild siRNA (As strand): | CUCACCGACUCCUCUACUGUAAAAAG
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ASP-siRNA (As strand): | CUCACCGA---CUCUACUGUAAAAAG
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Mismatch position in siRNA: | DeltaGAG |
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Mutant allele (5'->3'): | GAGUGGCU---GAGAUGACAUUUUUC
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ASP-siRNA (As strand): | CUCACCGA---CUCUACUGUAAAAAG
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ASP-siRNA efficacy for mutant allele: | 0
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Wild allele (5'->3'): | GAGUGGCUGAGGAGAUGACAUUUUUC
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ASP-siRNA (As strand): | CUCACCGA---CUCUACUGUAAAAAG
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ASP-siRNA efficacy for wild allele: | 27
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Percentage discrimination for ASP-siRNA: | -27
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Wild allele (5'->3'): | GAGUGGCUGAGGAGAUGACAUUUUUC
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Wild siRNA (As strand): | CUCACCGACUCCUCUACUGUAAAAAG
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Wild sirna efficacy for wild allele: | 0
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Wild allele (5'->3'): | GAGUGGCUGAGGAGAUGACAUUUUUC
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ASP-siRNA (As strand): | CUCACCGA---CUCUACUGUAAAAAG
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Wild sirna efficacy for mutant allele: | 82
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Percentage discrimination for wild siRNA: | -82
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| Concentration: | 5microg |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | O14656 |
| Target sequence: | GUAAGCAGAGUGGCUGAGGAGAUGACAUUUUUCCCCAAAGAG |
| Genbank Accesion: | NM_000113.2 |
| Cell-line: | Cos-7 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Epicentre Technologies |
| Post-transfection duration: | 36-48 hours |
| OMID ID: | 128100 |
| KEGG disease ID: | H01255 |
| Reference: | 12783425 |
