aspsirna0560 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0560
siRNA Name: R shRNA
Gene:DsRed2
Disease:Congenital myasthenic syndrome (SCCMS)
Wild allele/target (5'->3'): NA
Mutant allele/target (5'->3'):GAGAACGUCAUCACCGAGUU
Target Mutation: NA
Wild siRNA (As strand):NA
ASP-siRNA (As strand):CUCUUGCAGUAGUGGCUCAA
Mismatch position in siRNA: NA
Mutant allele (5'->3'): GAGAACGUCAUCACCGAGUU
ASP-siRNA (As strand):CUCUUGCAGUAGUGGCUCAA
ASP-siRNA efficacy for mutant allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand):CUCUUGCAGUAGUGGCUCAA
ASP-siRNA efficacy for wild allele:NA
Percentage discrimination for ASP-siRNA:NA
Wild allele (5'->3'): NA
Wild siRNA (As strand):NA
Wild sirna efficacy for wild allele:NA
Wild allele (5'->3'): NA
ASP-siRNA (As strand):CUCUUGCAGUAGUGGCUCAA
Wild sirna efficacy for mutant allele:4
Percentage discrimination for wild siRNA:NA
Concentration:100pmol
Orientation:Sense
Mismatch in:siRNA
UniProt ID: Q2WG74
Target sequence: GGATCCTGATGGCCTCCTCCGAGAACGTC
Genbank Accesion: DQ005468.1
Cell-line: HEK293
Experimental technique used: 125 I-a-bungarotoxin binding aSay
Transfection method: Polyethyleneimine
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
OMID ID: 608931
KEGG disease ID:H00770
Reference:12928480

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