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siRNA Id: | aspsirna0561
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siRNA Name: | R M9-shRNA
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| Gene: | DsRed2 |
| Disease: | Congenital myasthenic syndrome (SCCMS) |
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Wild allele/target (5'->3'): | GAGAACGUCAUCACCGAGUU
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Mutant allele/target (5'->3'): | GAGAACGUUAUCACCGAGUU
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Target Mutation: | NA
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Wild siRNA (As strand): | CUCUUGCAGUAGUGGCUCAA
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ASP-siRNA (As strand): | CUCUUGCAAUAGUGGCUCAA
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Mismatch position in siRNA: | A12G |
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Mutant allele (5'->3'): | GAGAACGUUAUCACCGAGUU
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ASP-siRNA (As strand): | CUCUUGCAAUAGUGGCUCAA
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ASP-siRNA efficacy for mutant allele: | 0
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Wild allele (5'->3'): | GAGAACGUCAUCACCGAGUU
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ASP-siRNA (As strand): | CUCUUGCAAUAGUGGCUCAA
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ASP-siRNA efficacy for wild allele: | 0
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Percentage discrimination for ASP-siRNA: | 0
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Wild allele (5'->3'): | GAGAACGUCAUCACCGAGUU
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Wild siRNA (As strand): | CUCUUGCAGUAGUGGCUCAA
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | GAGAACGUCAUCACCGAGUU
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ASP-siRNA (As strand): | CUCUUGCAAUAGUGGCUCAA
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Wild sirna efficacy for mutant allele: | 4
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 100pmol |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q2WG74 |
| Target sequence: | GGATCCTGATGGCCTCCTCCGAGAACGTC |
| Genbank Accesion: | DQ005468.1 |
| Cell-line: | HEK293 |
| Experimental technique used: | Flourescence microscopy |
| Transfection method: | Polyethyleneimine |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 120 hours |
| OMID ID: | 608931 |
| KEGG disease ID: | H00770 |
| Reference: | 12928480 |
