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siRNA Id: | aspsirna0573
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siRNA Name: | LM6
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| Gene: | Amyloid beta (A4) precursor protein(APP) |
| Disease: | Alzheimers disease |
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Wild allele/target (5'->3'): | CGCGACAGUGAUCGUCAUCACCUUG
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Mutant allele/target (5'->3'): | CGCGACAGUGAUCAUCAUCACCUUG
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Target Mutation: | c.2149G>A,p.Val717Ile
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Wild siRNA (As strand): | GCGCUGUCACUAGCAGUAGUGGAAC
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ASP-siRNA (As strand): | GCGCUGUCACUAGUAGUAGUGGAAC
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Mismatch position in siRNA: | U12C |
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Mutant allele (5'->3'): | CGCGACAGUGAUCAUCAUCACCUUG
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ASP-siRNA (As strand): | GCGCUGUCACUAGUAGUAGUGGAAC
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ASP-siRNA efficacy for mutant allele: | 20
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Wild allele (5'->3'): | CGCGACAGUGAUCGUCAUCACCUUG
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ASP-siRNA (As strand): | GCGCUGUCACUAGUAGUAGUGGAAC
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ASP-siRNA efficacy for wild allele: | 58
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Percentage discrimination for ASP-siRNA: | -38
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Wild allele (5'->3'): | CGCGACAGUGAUCGUCAUCACCUUG
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Wild siRNA (As strand): | GCGCUGUCACUAGCAGUAGUGGAAC
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CGCGACAGUGAUCGUCAUCACCUUG
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ASP-siRNA (As strand): | GCGCUGUCACUAGUAGUAGUGGAAC
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 100pmol |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P05067 |
| Target sequence: | AGCGACAGUGAUCGUCAUCACCU |
| Genbank Accesion: | NM_000484.3 |
| Cell-line: | CHO |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Epicentre Technologies |
| Post-transfection duration: | 48 hours |
| OMID ID: | 104300 |
| KEGG disease ID: | H00056 |
| Reference: | 16426772 |
