| |
|---|
|
siRNA Id: | aspsirna0574
|
|
siRNA Name: | LM2C
|
| Gene: | Amyloid beta (A4) precursor protein(APP) |
| Disease: | Alzheimers disease |
|
Wild allele/target (5'->3'): | CGACAGUGAUCGUCAUCACCUU
|
|
Mutant allele/target (5'->3'): | GGACAGUCAUCAUCAUCACCUU
|
|
Target Mutation: | c.2149G>A,p.Val717Ile
|
|
Wild siRNA (As strand): | GCUGUCACUAGCAGUAGUGGAA
|
|
ASP-siRNA (As strand): | CCUGUCAGUAGUAGUAGUGGAA
|
|
Mismatch position in siRNA: | U11C,G15C,C22G |
|
Mutant allele (5'->3'): | GGACAGUCAUCAUCAUCACCUU
|
|
ASP-siRNA (As strand): | CCUGUCAGUAGUAGUAGUGGAA
|
|
ASP-siRNA efficacy for mutant allele: | 29
|
|
Wild allele (5'->3'): | CGACAGUGAUCGUCAUCACCUU
|
|
ASP-siRNA (As strand): | CCUGUCAGUAGUAGUAGUGGAA
|
|
ASP-siRNA efficacy for wild allele: | 75
|
|
Percentage discrimination for ASP-siRNA: | -46
|
|
Wild allele (5'->3'): | CGACAGUGAUCGUCAUCACCUU
|
|
Wild siRNA (As strand): | GCUGUCACUAGCAGUAGUGGAA
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | CGACAGUGAUCGUCAUCACCUU
|
|
ASP-siRNA (As strand): | CCUGUCAGUAGUAGUAGUGGAA
|
|
Wild sirna efficacy for mutant allele: | NA
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 100pmol |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P05067 |
| Target sequence: | AGCGACAGUGAUCGUCAUCACCU |
| Genbank Accesion: | NM_000484.3 |
| Cell-line: | CHO |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Epicentre Technologies |
| Post-transfection duration: | 48 hours |
| OMID ID: | 104300 |
| KEGG disease ID: | H00056 |
| Reference: | 16426772 |
