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siRNA Id: | aspsirna0592
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siRNA Name: | siK9-p.R163W-17
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Gene: | Keratin 9 (KRT9) |
Disease: | Epidermolytic palmoplantar keratoderma (EPPK) |
Wild allele/target (5'->3'): | CUCGGCUGGCCUCUUACUU
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Mutant allele/target (5'->3'): | CUUGGCUGGCCUCUUACUU
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Target Mutation: | c.487C>T,p.Arg163Trp
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Wild siRNA (As strand): | GAGCCGACCGGAGAAUGAA
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ASP-siRNA (As strand): | GAACCGACCGGAGAAUGAA
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Mismatch position in siRNA: | A17G |
Mutant allele (5'->3'): | CUUGGCUGGCCUCUUACUU
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ASP-siRNA (As strand): | GAACCGACCGGAGAAUGAA
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ASP-siRNA efficacy for mutant allele: | 60
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Wild allele (5'->3'): | CUCGGCUGGCCUCUUACUU
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ASP-siRNA (As strand): | GAACCGACCGGAGAAUGAA
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ASP-siRNA efficacy for wild allele: | 0
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Percentage discrimination for ASP-siRNA: | 60
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Wild allele (5'->3'): | CUCGGCUGGCCUCUUACUU
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Wild siRNA (As strand): | GAGCCGACCGGAGAAUGAA
|
Wild sirna efficacy for wild allele: | 67
|
Wild allele (5'->3'): | CUCGGCUGGCCUCUUACUU
|
ASP-siRNA (As strand): | GAACCGACCGGAGAAUGAA
|
Wild sirna efficacy for mutant allele: | 30
|
Percentage discrimination for wild siRNA: | 37
|
Concentration: | 0-6.25nM |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | P35527 |
Target sequence: | CCATGCAGGAACTCAATTCT*C#GGCTGGCCTCTTACTTGGATA |
Genbank Accesion: | NM_000226.3 |
Cell-line: | AD293 |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Dharmacon |
Post-transfection duration: | 24 hours |
OMID ID: | 144200 |
KEGG disease ID: | H00722 |
Reference: | 22402445 |