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siRNA Id: | aspsirna0593
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siRNA Name: | siK9-p.R163W-18
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| Gene: | Keratin 9 (KRT9) |
| Disease: | Epidermolytic palmoplantar keratoderma (EPPK) |
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Wild allele/target (5'->3'): | UCGGCUGGCCUCUUACUUG
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Mutant allele/target (5'->3'): | UUGGCUGGCCUCUUACUUG
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Target Mutation: | c.487C>T,p.Arg163Trp
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Wild siRNA (As strand): | AGCCGACCGGAGAAUGAAC
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ASP-siRNA (As strand): | AACCGACCGGAGAAUGAAC
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Mismatch position in siRNA: | A18G |
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Mutant allele (5'->3'): | UUGGCUGGCCUCUUACUUG
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ASP-siRNA (As strand): | AACCGACCGGAGAAUGAAC
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ASP-siRNA efficacy for mutant allele: | 55
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Wild allele (5'->3'): | UCGGCUGGCCUCUUACUUG
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ASP-siRNA (As strand): | AACCGACCGGAGAAUGAAC
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ASP-siRNA efficacy for wild allele: | 15
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Percentage discrimination for ASP-siRNA: | 40
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Wild allele (5'->3'): | UCGGCUGGCCUCUUACUUG
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Wild siRNA (As strand): | AGCCGACCGGAGAAUGAAC
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Wild sirna efficacy for wild allele: | 67
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Wild allele (5'->3'): | UCGGCUGGCCUCUUACUUG
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ASP-siRNA (As strand): | AACCGACCGGAGAAUGAAC
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Wild sirna efficacy for mutant allele: | 30
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Percentage discrimination for wild siRNA: | 37
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| Concentration: | 0-6.25nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P35527 |
| Target sequence: | CCATGCAGGAACTCAATTCT*C#GGCTGGCCTCTTACTTGGATA |
| Genbank Accesion: | NM_000226.3 |
| Cell-line: | AD293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 24 hours |
| OMID ID: | 144200 |
| KEGG disease ID: | H00722 |
| Reference: | 22402445 |
