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siRNA Id: | aspsirna0655
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siRNA Name: | SiRNA-A16
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| Gene: | Potasium channel voltage-gated subfamily h member 2(KCNH2) |
| Disease: | Long QT Syndrome (LQTS) |
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Wild allele/target (5'->3'): | CUACUCGGCUGCCUUCCUG
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Mutant allele/target (5'->3'): | CUAUUCGGCUGCCUUCCUG
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Target Mutation: | c.1682C>T ,p.Ala561Val
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Wild siRNA (As strand): | GAUGAGCCGACGGAAGGAC
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ASP-siRNA (As strand): | GAUAAGCCGACGGAAGGAC
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Mismatch position in siRNA: | A16G |
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Mutant allele (5'->3'): | CUAUUCGGCUGCCUUCCUG
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ASP-siRNA (As strand): | GAUAAGCCGACGGAAGGAC
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ASP-siRNA efficacy for mutant allele: | 10
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Wild allele (5'->3'): | CUACUCGGCUGCCUUCCUG
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ASP-siRNA (As strand): | GAUAAGCCGACGGAAGGAC
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ASP-siRNA efficacy for wild allele: | 78
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Percentage discrimination for ASP-siRNA: | -68
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Wild allele (5'->3'): | CUACUCGGCUGCCUUCCUG
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Wild siRNA (As strand): | GAUGAGCCGACGGAAGGAC
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CUACUCGGCUGCCUUCCUG
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ASP-siRNA (As strand): | GAUAAGCCGACGGAAGGAC
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 20microM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q12809 |
| Target sequence: | ACTCGGCTGCCTTCCTGCTGAAGGAGACGG |
| Genbank Accesion: | NM_001204798.1 |
| Cell-line: | cardiomyocytes |
| Experimental technique used: | RT-PCR, Flourescence microscopy |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 72 hours |
| OMID ID: | 152427 |
| KEGG disease ID: | H00720 |
| Reference: | 23470493 |
