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siRNA Id: | aspsirna0660
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siRNA Name: | P4
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| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
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Wild allele/target (5'->3'): | CAGCAGCAGCAGCAGCAGC
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Mutant allele/target (5'->3'): | CAGCAGCAGCAGCAGUAGC
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Target Mutation: | c.52CAG(36_39)
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Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCG
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ASP-siRNA (As strand): | GUCGUCGUCGUCGUCAUCG
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Mismatch position in siRNA: | A4G |
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Mutant allele (5'->3'): | CAGCAGCAGCAGCAGUAGC
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ASP-siRNA (As strand): | GUCGUCGUCGUCGUCAUCG
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ASP-siRNA efficacy for mutant allele: | 20
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
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ASP-siRNA (As strand): | GUCGUCGUCGUCGUCAUCG
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ASP-siRNA efficacy for wild allele: | 13
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Percentage discrimination for ASP-siRNA: | 7
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
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Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCG
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Wild sirna efficacy for wild allele: | Lowest effective
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
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ASP-siRNA (As strand): | GUCGUCGUCGUCGUCAUCG
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Wild sirna efficacy for mutant allele: | 0
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Percentage discrimination for wild siRNA: | Poor discrimination
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| Concentration: | 50nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q96CV9 |
| Target sequence: | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC |
| Genbank Accesion: | NM_002111.7 |
| Cell-line: | Fibroblast |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiMAX |
| siRNA expression method: | Integrated DNA Technologies |
| Post-transfection duration: | 72 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H01243 |
| Reference: | 23042244 |
