| |
|---|
|
siRNA Id: | aspsirna0682
|
|
siRNA Name: | P16
|
| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
|
Wild allele/target (5'->3'): | CAGCAGCAGCAGCAGCAGCAGC
|
|
Mutant allele/target (5'->3'): | CAGCAGCAGCAGCAGAAGCAGC
|
|
Target Mutation: | c.52CAG(36_39)
|
|
Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCGUCG
|
|
ASP-siRNA (As strand): | GUCGUCGUCGUCGUCUUCGUCG
|
|
Mismatch position in siRNA: | U7G |
|
Mutant allele (5'->3'): | CAGCAGCAGCAGCAGAAGCAGC
|
|
ASP-siRNA (As strand): | GUCGUCGUCGUCGUCUUCGUCG
|
|
ASP-siRNA efficacy for mutant allele: | 50
|
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAGC
|
|
ASP-siRNA (As strand): | GUCGUCGUCGUCGUCUUCGUCG
|
|
ASP-siRNA efficacy for wild allele: | 30
|
|
Percentage discrimination for ASP-siRNA: | 20
|
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAGC
|
|
Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCGUCG
|
|
Wild sirna efficacy for wild allele: | Moderately effective
|
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAGC
|
|
ASP-siRNA (As strand): | GUCGUCGUCGUCGUCUUCGUCG
|
|
Wild sirna efficacy for mutant allele: | 20
|
|
Percentage discrimination for wild siRNA: | High discrimination
|
| Concentration: | 3microliter |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q96CV9 |
| Target sequence: | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC |
| Genbank Accesion: | NM_002111.7 |
| Cell-line: | Fibroblast |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiMAX |
| siRNA expression method: | Isis Pharmaceuticals |
| Post-transfection duration: | 4 days |
| OMID ID: | 143100 |
| KEGG disease ID: | H00059 |
| Reference: | 22939619 |
