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siRNA Id: | aspsirna0717
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siRNA Name: | P13
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Gene: | Cu,Zn superoxide dismutase (SOD1) |
Disease: | Amyotrophic Lateral Sclerosis (ALS) |
Wild allele/target (5'->3'): | GACUUGGGCAAUGUGACUG
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Mutant allele/target (5'->3'): | GACUUGCGCAAUGUGACUG
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Target Mutation: | c.256G>C, p.Gly86Arg
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Wild siRNA (As strand): | CUGAACCCGUUACACUGAC
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ASP-siRNA (As strand): | CUGAACGCGUUACACUGAC
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Mismatch position in siRNA: | G13C |
Mutant allele (5'->3'): | GACUUGCGCAAUGUGACUG
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ASP-siRNA (As strand): | CUGAACGCGUUACACUGAC
|
ASP-siRNA efficacy for mutant allele: | 62
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Wild allele (5'->3'): | GACUUGGGCAAUGUGACUG
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ASP-siRNA (As strand): | CUGAACGCGUUACACUGAC
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ASP-siRNA efficacy for wild allele: | 28
|
Percentage discrimination for ASP-siRNA: | 34
|
Wild allele (5'->3'): | GACUUGGGCAAUGUGACUG
|
Wild siRNA (As strand): | CUGAACCCGUUACACUGAC
|
Wild sirna efficacy for wild allele: | NA
|
Wild allele (5'->3'): | GACUUGGGCAAUGUGACUG
|
ASP-siRNA (As strand): | CUGAACGCGUUACACUGAC
|
Wild sirna efficacy for mutant allele: | High efficacy
|
Percentage discrimination for wild siRNA: | 18
|
Concentration: | 2nM |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | P00441 |
Target sequence: | TGAAGAGAGGCATGTTGGAGACTTGGGCAATGTGAC |
Genbank Accesion: | NM_000454.4 |
Cell-line: | Drosophila embryo lysate |
Experimental technique used: | Flourescence reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Chemically synthesized |
Post-transfection duration: | 24 hours |
OMID ID: | 105400 |
KEGG disease ID: | H00058 |
Reference: | 16965178 |