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siRNA Id: | aspsirna0746
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siRNA Name: | P3
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| Gene: | Cu,Zn superoxide dismutase (SOD1) |
| Disease: | Amyotrophic Lateral Sclerosis (ALS) |
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Wild allele/target (5'->3'): | GCAUGUUGGAGACUUGUGC
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Mutant allele/target (5'->3'): | GCAUGUUGGAGACUUGCGC
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Target Mutation: | c.256G>C, p.Gly86Arg
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Wild siRNA (As strand): | CGUACAACCUCUGAACACG
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ASP-siRNA (As strand): | CGUACAACCUCUGAACGCG
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Mismatch position in siRNA: | G3A |
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Mutant allele (5'->3'): | GCAUGUUGGAGACUUGCGC
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ASP-siRNA (As strand): | CGUACAACCUCUGAACGCG
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ASP-siRNA efficacy for mutant allele: | 92
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Wild allele (5'->3'): | GCAUGUUGGAGACUUGUGC
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ASP-siRNA (As strand): | CGUACAACCUCUGAACGCG
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ASP-siRNA efficacy for wild allele: | 38
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Percentage discrimination for ASP-siRNA: | 54
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Wild allele (5'->3'): | GCAUGUUGGAGACUUGUGC
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Wild siRNA (As strand): | CGUACAACCUCUGAACACG
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | GCAUGUUGGAGACUUGUGC
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ASP-siRNA (As strand): | CGUACAACCUCUGAACGCG
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Wild sirna efficacy for mutant allele: | High efficacy
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Percentage discrimination for wild siRNA: | 18
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| Concentration: | 100nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P00441 |
| Target sequence: | TGAAGAGAGGCATGTTGGAGACTTGGGCAATGTGAC |
| Genbank Accesion: | NM_000454.4 |
| Cell-line: | HeLa |
| Experimental technique used: | Flourescence reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 24 hours |
| OMID ID: | 105400 |
| KEGG disease ID: | H00058 |
| Reference: | 16965178 |
