| |
|---|
|
siRNA Id: | aspsirna0773
|
|
siRNA Name: | P10U
|
| Gene: | Cu,Zn superoxide dismutase (SOD1) |
| Disease: | Amyotrophic Lateral Sclerosis (ALS) |
|
Wild allele/target (5'->3'): | GGAGACUUGGGCAAUGUGA
|
|
Mutant allele/target (5'->3'): | GUGGUUGGUGAAGCCUCAG
|
|
Target Mutation: | c.256G>C, p.Gly86Arg
|
|
Wild siRNA (As strand): | CCUCUGAACCCGUUACACU
|
|
ASP-siRNA (As strand): | CACCAACCAUUUCGGAGUC
|
|
Mismatch position in siRNA: | U10C |
|
Mutant allele (5'->3'): | GUGGUUGGUGAAGCCUCAG
|
|
ASP-siRNA (As strand): | CACCAACCAUUUCGGAGUC
|
|
ASP-siRNA efficacy for mutant allele: | 65
|
|
Wild allele (5'->3'): | GGAGACUUGGGCAAUGUGA
|
|
ASP-siRNA (As strand): | CACCAACCAUUUCGGAGUC
|
|
ASP-siRNA efficacy for wild allele: | 11
|
|
Percentage discrimination for ASP-siRNA: | 54
|
|
Wild allele (5'->3'): | GGAGACUUGGGCAAUGUGA
|
|
Wild siRNA (As strand): | CCUCUGAACCCGUUACACU
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | GGAGACUUGGGCAAUGUGA
|
|
ASP-siRNA (As strand): | CACCAACCAUUUCGGAGUC
|
|
Wild sirna efficacy for mutant allele: | Moderate efficacy
|
|
Percentage discrimination for wild siRNA: | 45
|
| Concentration: | 2nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P00441 |
| Target sequence: | TGAAGAGAGGCATGTTGGAGACTTGGGCAATGTGAC |
| Genbank Accesion: | NM_000454.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Flourescence reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 24 hours |
| OMID ID: | 105400 |
| KEGG disease ID: | H00058 |
| Reference: | 16965178 |
