aspsirna0789 details

Purple = Wild vs mutant target sequence along with mutation information
Brown= Wild vs ASP-siRNA description along with positional differnece between two
Light pink= ASP siRNA efficacy for mutant vs wild allele
Green= Wild siRNA efficacy for Wild vs Mutant allele
siRNA Id:aspsirna0789
siRNA Name: PM4
Gene:Hungtintin (HTT)
Disease:Huntington disease (HD)
Wild allele/target (5'->3'): CAGCAGCAGCAGCAGCAGC
Mutant allele/target (5'->3'):CAGCAGCAUUUUCAGCAGC
Target Mutation: c.52CAG(36_39)
Wild siRNA (As strand):GUCGUCGUCGUCGUCGUCG
ASP-siRNA (As strand):GUCGUCGUAAAAGUCGUCG
Mismatch position in siRNA: A8C,A9U,A10G,A11C
Mutant allele (5'->3'): CAGCAGCAUUUUCAGCAGC
ASP-siRNA (As strand):GUCGUCGUAAAAGUCGUCG
ASP-siRNA efficacy for mutant allele:78
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGC
ASP-siRNA (As strand):GUCGUCGUAAAAGUCGUCG
ASP-siRNA efficacy for wild allele:25
Percentage discrimination for ASP-siRNA:53
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGC
Wild siRNA (As strand):GUCGUCGUCGUCGUCGUCG
Wild sirna efficacy for wild allele:72
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGC
ASP-siRNA (As strand):GUCGUCGUAAAAGUCGUCG
Wild sirna efficacy for mutant allele:50
Percentage discrimination for wild siRNA:22
Concentration:25nM
Orientation:Sense
Mismatch in:siRNA
UniProt ID: Q96CV9
Target sequence: CAGCAGCAGCAGCAGCAGCAGCAGCAGC
Genbank Accesion: NM_002111.7
Cell-line: Fibroblast
Experimental technique used: Western blot
Transfection method: RNAiMAX
siRNA expression method: Integrated DNA Technologies
Post-transfection duration: 72 hours
OMID ID: 143100
KEGG disease ID:H00059
Reference:21095568

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