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siRNA Id: | aspsirna0789
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siRNA Name: | PM4
|
Gene: | Hungtintin (HTT) |
Disease: | Huntington disease (HD) |
Wild allele/target (5'->3'): | CAGCAGCAGCAGCAGCAGC
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Mutant allele/target (5'->3'): | CAGCAGCAUUUUCAGCAGC
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Target Mutation: | c.52CAG(36_39)
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Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCG
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ASP-siRNA (As strand): | GUCGUCGUAAAAGUCGUCG
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Mismatch position in siRNA: | A8C,A9U,A10G,A11C |
Mutant allele (5'->3'): | CAGCAGCAUUUUCAGCAGC
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ASP-siRNA (As strand): | GUCGUCGUAAAAGUCGUCG
|
ASP-siRNA efficacy for mutant allele: | 78
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
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ASP-siRNA (As strand): | GUCGUCGUAAAAGUCGUCG
|
ASP-siRNA efficacy for wild allele: | 25
|
Percentage discrimination for ASP-siRNA: | 53
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
|
Wild siRNA (As strand): | GUCGUCGUCGUCGUCGUCG
|
Wild sirna efficacy for wild allele: | 72
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGC
|
ASP-siRNA (As strand): | GUCGUCGUAAAAGUCGUCG
|
Wild sirna efficacy for mutant allele: | 50
|
Percentage discrimination for wild siRNA: | 22
|
Concentration: | 25nM |
Orientation: | Sense |
Mismatch in: | siRNA |
UniProt ID: | Q96CV9 |
Target sequence: | CAGCAGCAGCAGCAGCAGCAGCAGCAGC |
Genbank Accesion: | NM_002111.7 |
Cell-line: | Fibroblast |
Experimental technique used: | Western blot |
Transfection method: | RNAiMAX |
siRNA expression method: | Integrated DNA Technologies |
Post-transfection duration: | 72 hours |
OMID ID: | 143100 |
KEGG disease ID: | H00059 |
Reference: | 21095568 |