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siRNA Id: | aspsirna0803
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siRNA Name: | SHP2
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| Gene: | Cu,Zn superoxide dismutase (SOD1) |
| Disease: | Amyotrophic Lateral Sclerosis (ALS) |
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Wild allele/target (5'->3'): | UGGAUUCCAUGUUCAUGAG
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Mutant allele/target (5'->3'): | UCGAUUCCAUGUUCAUGAG
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Target Mutation: | c.281G>C,p.Gly94Ala
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Wild siRNA (As strand): | ACCUAAGGUACAAGUACUC
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ASP-siRNA (As strand): | AGCUAAGGUACAAGUACUC
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Mismatch position in siRNA: | G18C |
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Mutant allele (5'->3'): | UCGAUUCCAUGUUCAUGAG
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ASP-siRNA (As strand): | AGCUAAGGUACAAGUACUC
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ASP-siRNA efficacy for mutant allele: | 50
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Wild allele (5'->3'): | UGGAUUCCAUGUUCAUGAG
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ASP-siRNA (As strand): | AGCUAAGGUACAAGUACUC
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ASP-siRNA efficacy for wild allele: | 42
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Percentage discrimination for ASP-siRNA: | 8
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Wild allele (5'->3'): | UGGAUUCCAUGUUCAUGAG
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Wild siRNA (As strand): | ACCUAAGGUACAAGUACUC
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | UGGAUUCCAUGUUCAUGAG
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ASP-siRNA (As strand): | AGCUAAGGUACAAGUACUC
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 300nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P00441 |
| Target sequence: | GGATTCCATGTTCATGAGTTTGGAGATAA |
| Genbank Accesion: | NM_000454.4 |
| Cell-line: | HEK293 |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiMAX |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 168 hours |
| OMID ID: | 105400 |
| KEGG disease ID: | H00058 |
| Reference: | 15768029 |
