| |
|---|
|
siRNA Id: | aspsirna0811
|
|
siRNA Name: | P7_rs362307
|
| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
|
Wild allele/target (5'->3'): | CCUUUGGAAGUCUGCGCCCUA
|
|
Mutant allele/target (5'->3'): | CCUUUGGAAGUCUGUGCCCUA
|
|
Target Mutation: | c.52CAG(36_39)
|
|
Wild siRNA (As strand): | GGAAACCUUCAGACGCGGGAU
|
|
ASP-siRNA (As strand): | GGAAACCUUCAGACACGGGAU
|
|
Mismatch position in siRNA: | A7G |
|
Mutant allele (5'->3'): | CCUUUGGAAGUCUGUGCCCUA
|
|
ASP-siRNA (As strand): | GGAAACCUUCAGACACGGGAU
|
|
ASP-siRNA efficacy for mutant allele: | 62
|
|
Wild allele (5'->3'): | CCUUUGGAAGUCUGCGCCCUA
|
|
ASP-siRNA (As strand): | GGAAACCUUCAGACACGGGAU
|
|
ASP-siRNA efficacy for wild allele: | 43
|
|
Percentage discrimination for ASP-siRNA: | 19
|
|
Wild allele (5'->3'): | CCUUUGGAAGUCUGCGCCCUA
|
|
Wild siRNA (As strand): | GGAAACCUUCAGACGCGGGAU
|
|
Wild sirna efficacy for wild allele: | 40
|
|
Wild allele (5'->3'): | CCUUUGGAAGUCUGCGCCCUA
|
|
ASP-siRNA (As strand): | GGAAACCUUCAGACACGGGAU
|
|
Wild sirna efficacy for mutant allele: | 12
|
|
Percentage discrimination for wild siRNA: | 28
|
| Concentration: | 20nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q96CV9 |
| Target sequence: | GTCTGCGCCCTTGTGCCCTGCCTCCA |
| Genbank Accesion: | NM_002111.7 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H00059 |
| Reference: | 19361997 |
