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siRNA Id: | aspsirna0820
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siRNA Name: | P6+P10_rs362307
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| Gene: | Hungtintin (HTT) |
| Disease: | Huntington disease (HD) |
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Wild allele/target (5'->3'): | UUGGAAGUCUGCGCCCUUGUG
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Mutant allele/target (5'->3'): | UUGGAAGUCUGUGCCAUUGUG
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Target Mutation: | c.52CAG(36_39)
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Wild siRNA (As strand): | AACCUUCAGACGCGGGAACAC
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ASP-siRNA (As strand): | AACCUUCAGACACGGUAACAC
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Mismatch position in siRNA: | U6G,A10G |
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Mutant allele (5'->3'): | UUGGAAGUCUGUGCCAUUGUG
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ASP-siRNA (As strand): | AACCUUCAGACACGGUAACAC
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ASP-siRNA efficacy for mutant allele: | 57
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Wild allele (5'->3'): | UUGGAAGUCUGCGCCCUUGUG
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ASP-siRNA (As strand): | AACCUUCAGACACGGUAACAC
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ASP-siRNA efficacy for wild allele: | 30
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Percentage discrimination for ASP-siRNA: | 27
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Wild allele (5'->3'): | UUGGAAGUCUGCGCCCUUGUG
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Wild siRNA (As strand): | AACCUUCAGACGCGGGAACAC
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Wild sirna efficacy for wild allele: | 40
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Wild allele (5'->3'): | UUGGAAGUCUGCGCCCUUGUG
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ASP-siRNA (As strand): | AACCUUCAGACACGGUAACAC
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Wild sirna efficacy for mutant allele: | 12
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Percentage discrimination for wild siRNA: | 28
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| Concentration: | 20nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | Q96CV9 |
| Target sequence: | GTCTGCGCCCTTGTGCCCTGCCTCCA |
| Genbank Accesion: | NM_002111.7 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 143100 |
| KEGG disease ID: | H00059 |
| Reference: | 19361997 |
