| |
|---|
|
siRNA Id: | aspsirna0854
|
|
siRNA Name: | ShKras (G12D) 1
|
| Gene: | K-RAS (KRAS) |
| Disease: | Pancreatic cancer |
|
Wild allele/target (5'->3'): | GGCGGCGGCAGTGGCGGCGGC
|
|
Mutant allele/target (5'->3'): | AGCGGCGGCAGTGGCGGCGGC
|
|
Target Mutation: | c.36G>A,p.Gly12Asp
|
|
Wild siRNA (As strand): | CCGCCGCCGUCTCCGCCGCCG
|
|
ASP-siRNA (As strand): | UCGCCGCCGUCTCCGCCGCCG
|
|
Mismatch position in siRNA: | U19C |
|
Mutant allele (5'->3'): | AGCGGCGGCAGTGGCGGCGGC
|
|
ASP-siRNA (As strand): | UCGCCGCCGUCTCCGCCGCCG
|
|
ASP-siRNA efficacy for mutant allele: | 65
|
|
Wild allele (5'->3'): | GGCGGCGGCAGTGGCGGCGGC
|
|
ASP-siRNA (As strand): | UCGCCGCCGUCTCCGCCGCCG
|
|
ASP-siRNA efficacy for wild allele: | 25
|
|
Percentage discrimination for ASP-siRNA: | 40
|
|
Wild allele (5'->3'): | GGCGGCGGCAGTGGCGGCGGC
|
|
Wild siRNA (As strand): | CCGCCGCCGUCTCCGCCGCCG
|
|
Wild sirna efficacy for wild allele: | NA
|
|
Wild allele (5'->3'): | GGCGGCGGCAGTGGCGGCGGC
|
|
ASP-siRNA (As strand): | UCGCCGCCGUCTCCGCCGCCG
|
|
Wild sirna efficacy for mutant allele: | NA
|
|
Percentage discrimination for wild siRNA: | NA
|
| Concentration: | 10ml |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P01116 |
| Target sequence: | CGGCGGCGGCAGTGGCGGCGGCGAA |
| Genbank Accesion: | NM_033360.3 |
| Cell-line: | ASPC1 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 260350 |
| KEGG disease ID: | H00019 |
| Reference: | 21364589 |
