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siRNA Id: | aspsirna0855
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siRNA Name: | ShKras (G12D) 2
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| Gene: | K-RAS (KRAS) |
| Disease: | Pancreatic cancer |
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Wild allele/target (5'->3'): | CGGCGGCGGCAGTGGCGGC
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Mutant allele/target (5'->3'): | CAGCGGCGGCAGTGGCGGC
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Target Mutation: | c.36G>A,p.Gly12Asp
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Wild siRNA (As strand): | GCCGCCGCCGUCTCCGCCG
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ASP-siRNA (As strand): | GUCGCCGCCGUCTCCGCCG
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Mismatch position in siRNA: | U18C |
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Mutant allele (5'->3'): | CAGCGGCGGCAGTGGCGGC
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ASP-siRNA (As strand): | GUCGCCGCCGUCTCCGCCG
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ASP-siRNA efficacy for mutant allele: | 70
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Wild allele (5'->3'): | CGGCGGCGGCAGTGGCGGC
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ASP-siRNA (As strand): | GUCGCCGCCGUCTCCGCCG
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ASP-siRNA efficacy for wild allele: | 44
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Percentage discrimination for ASP-siRNA: | 26
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Wild allele (5'->3'): | CGGCGGCGGCAGTGGCGGC
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Wild siRNA (As strand): | GCCGCCGCCGUCTCCGCCG
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CGGCGGCGGCAGTGGCGGC
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ASP-siRNA (As strand): | GUCGCCGCCGUCTCCGCCG
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 10ml |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P01116 |
| Target sequence: | CGGCGGCGGCAGTGGCGGCGGCGAA |
| Genbank Accesion: | NM_033360.3 |
| Cell-line: | ASPC1 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| OMID ID: | 260350 |
| KEGG disease ID: | H00019 |
| Reference: | 21364589 |
