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siRNA Id: | aspsirna0858
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siRNA Name: | ShR-P12
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| Gene: | Ataxin-7(ATXN7) |
| Disease: | Spino Cerebellar Ataxia type-7 (SCA-7) |
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Wild allele/target (5'->3'): | GCCAGCCGUGAACAAUGUC
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Mutant allele/target (5'->3'): | GCCAGCCAUGAACAAUGUC
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Target Mutation: | c.88_90CAG(7_17)
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Wild siRNA (As strand): | CGGUCGGCACUUGUUACAG
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ASP-siRNA (As strand): | CGGUCGGUACUUGUUACAG
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Mismatch position in siRNA: | U12C |
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Mutant allele (5'->3'): | GCCAGCCAUGAACAAUGUC
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ASP-siRNA (As strand): | CGGUCGGUACUUGUUACAG
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ASP-siRNA efficacy for mutant allele: | 83
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Wild allele (5'->3'): | GCCAGCCGUGAACAAUGUC
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ASP-siRNA (As strand): | CGGUCGGUACUUGUUACAG
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ASP-siRNA efficacy for wild allele: | 30
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Percentage discrimination for ASP-siRNA: | 53
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Wild allele (5'->3'): | GCCAGCCGUGAACAAUGUC
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Wild siRNA (As strand): | CGGUCGGCACUUGUUACAG
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Wild sirna efficacy for wild allele: | 49
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Wild allele (5'->3'): | GCCAGCCGUGAACAAUGUC
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ASP-siRNA (As strand): | CGGUCGGUACUUGUUACAG
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Wild sirna efficacy for mutant allele: | 20
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Percentage discrimination for wild siRNA: | 29
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| Concentration: | 50nM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | O15265 |
| Target sequence: | AGGUUGCCAAAGUGCCAGCCGUGAACAAUGUCCACAUGA |
| Genbank Accesion: | NM000333.3 |
| Cell-line: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Constructed by PCR |
| Post-transfection duration: | 48 hours |
| OMID ID: | 164500 |
| KEGG disease ID: | H00063 |
| Reference: | 19789634 |
