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siRNA Id: | aspsirna0872
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siRNA Name: | siP1
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| Gene: | Presenilin1 (PSEN1) |
| Disease: | Alzheimers disease |
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Wild allele/target (5'->3'): | CUCCCGGCAGGUGGUGGAGC
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Mutant allele/target (5'->3'): | GUCCCGGCAGGUGGUGGAGC
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Target Mutation: | c.458C>G, p.Leu153Val
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Wild siRNA (As strand): | GAGGGCCGUCCACCACCUCG
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ASP-siRNA (As strand): | CAGGGCCGUCCACCACCUCG
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Mismatch position in siRNA: | C19G |
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Mutant allele (5'->3'): | GUCCCGGCAGGUGGUGGAGC
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ASP-siRNA (As strand): | CAGGGCCGUCCACCACCUCG
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ASP-siRNA efficacy for mutant allele: | 60
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Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
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ASP-siRNA (As strand): | CAGGGCCGUCCACCACCUCG
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ASP-siRNA efficacy for wild allele: | 15
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Percentage discrimination for ASP-siRNA: | 45
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Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
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Wild siRNA (As strand): | GAGGGCCGUCCACCACCUCG
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Wild sirna efficacy for wild allele: | NA
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Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
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ASP-siRNA (As strand): | CAGGGCCGUCCACCACCUCG
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Wild sirna efficacy for mutant allele: | NA
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Percentage discrimination for wild siRNA: | NA
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| Concentration: | 100microM |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P49768 |
| Target sequence: | GGTAACTCCCGGCAGGTGGTGGAGCA |
| Genbank Accesion: | NM_000021.3 |
| Cell-line: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine |
| siRNA expression method: | Constructed by PCR |
| Post-transfection duration: | NA |
| OMID ID: | 104300 |
| KEGG disease ID: | H00056 |
| Reference: | 18776243 |
