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siRNA Id: | aspsirna0880
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siRNA Name: | K6a_513a.5
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| Gene: | Keratin 6a (KRT6a) |
| Disease: | Pachyonychia congenita (PC) |
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Wild allele/target (5'->3'): | AUCAAGACCCUCAACAACA
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Mutant allele/target (5'->3'): | AUCAAGACCCUCAAAAACA
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Target Mutation: | c.513C>A,p.Asn171Lys
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Wild siRNA (As strand): | UAGUUCUGGGAGUUGUUGU
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ASP-siRNA (As strand): | UAGUUCUGGGAGUUUUUGU
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Mismatch position in siRNA: | U5C |
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Mutant allele (5'->3'): | AUCAAGACCCUCAAAAACA
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ASP-siRNA (As strand): | UAGUUCUGGGAGUUUUUGU
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ASP-siRNA efficacy for mutant allele: | 67
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Wild allele (5'->3'): | AUCAAGACCCUCAACAACA
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ASP-siRNA (As strand): | UAGUUCUGGGAGUUUUUGU
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ASP-siRNA efficacy for wild allele: | 39
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Percentage discrimination for ASP-siRNA: | 28
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Wild allele (5'->3'): | AUCAAGACCCUCAACAACA
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Wild siRNA (As strand): | UAGUUCUGGGAGUUGUUGU
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Wild sirna efficacy for wild allele: | 46
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Wild allele (5'->3'): | AUCAAGACCCUCAACAACA
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ASP-siRNA (As strand): | UAGUUCUGGGAGUUUUUGU
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Wild sirna efficacy for mutant allele: | 12
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Percentage discrimination for wild siRNA: | 34
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| Concentration: | 150ng |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P02538 |
| Target sequence: | AACAGATCAAGACCCTCAACAACAAGTTTGCCTC |
| Genbank Accesion: | NM_005554.3 |
| Cell-line: | 293T |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 48 hours |
| OMID ID: | 167200 |
| KEGG disease ID: | H00684 |
| Reference: | 17914454 |
