| |
|---|
|
siRNA Id: | aspsirna0894
|
|
siRNA Name: | K6a_513a.19
|
| Gene: | Keratin 6a (KRT6a) |
| Disease: | Pachyonychia congenita (PC) |
|
Wild allele/target (5'->3'): | CAACAAGUUUGCCUCCUUC
|
|
Mutant allele/target (5'->3'): | AAACAAGUUUGCCUCCUUC
|
|
Target Mutation: | c.513C>A,p.Asn171Lys
|
|
Wild siRNA (As strand): | GUUGUUCAAACGGAGGAAG
|
|
ASP-siRNA (As strand): | UUUGUUCAAACGGAGGAAG
|
|
Mismatch position in siRNA: | U19C |
|
Mutant allele (5'->3'): | AAACAAGUUUGCCUCCUUC
|
|
ASP-siRNA (As strand): | UUUGUUCAAACGGAGGAAG
|
|
ASP-siRNA efficacy for mutant allele: | 34
|
|
Wild allele (5'->3'): | CAACAAGUUUGCCUCCUUC
|
|
ASP-siRNA (As strand): | UUUGUUCAAACGGAGGAAG
|
|
ASP-siRNA efficacy for wild allele: | 9
|
|
Percentage discrimination for ASP-siRNA: | 25
|
|
Wild allele (5'->3'): | CAACAAGUUUGCCUCCUUC
|
|
Wild siRNA (As strand): | GUUGUUCAAACGGAGGAAG
|
|
Wild sirna efficacy for wild allele: | 46
|
|
Wild allele (5'->3'): | CAACAAGUUUGCCUCCUUC
|
|
ASP-siRNA (As strand): | UUUGUUCAAACGGAGGAAG
|
|
Wild sirna efficacy for mutant allele: | 12
|
|
Percentage discrimination for wild siRNA: | 34
|
| Concentration: | 150ng |
| Orientation: | Sense |
| Mismatch in: | siRNA |
| UniProt ID: | P02538 |
| Target sequence: | AACAGATCAAGACCCTCAACAACAAGTTTGCCTC |
| Genbank Accesion: | NM_005554.3 |
| Cell-line: | 293T |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 48 hours |
| OMID ID: | 167200 |
| KEGG disease ID: | H00684 |
| Reference: | 17914454 |
