| |
|---|
|
siRNA Id: | aspsirna0907
|
|
siRNA Name: | Sibeta2m04
|
| Gene: | Beta 2-microglobulin (B2M) |
| Disease: | HLA incompatibility |
|
Wild allele/target (5'->3'): | UUCGAAGCUUGAAGGUAA
|
|
Mutant allele/target (5'->3'): | UUCGAAGCUUGAAGCUAA
|
|
Target Mutation: | c.31G>C,p.Ala11Pro
|
|
Wild siRNA (As strand): | AAGCUUCGAACUUCCAUU
|
|
ASP-siRNA (As strand): | AAGCUUCGAACUUCGAUU
|
|
Mismatch position in siRNA: | G4C |
|
Mutant allele (5'->3'): | UUCGAAGCUUGAAGCUAA
|
|
ASP-siRNA (As strand): | AAGCUUCGAACUUCGAUU
|
|
ASP-siRNA efficacy for mutant allele: | 89
|
|
Wild allele (5'->3'): | UUCGAAGCUUGAAGGUAA
|
|
ASP-siRNA (As strand): | AAGCUUCGAACUUCGAUU
|
|
ASP-siRNA efficacy for wild allele: | NA
|
|
Percentage discrimination for ASP-siRNA: | NA
|
|
Wild allele (5'->3'): | UUCGAAGCUUGAAGGUAA
|
|
Wild siRNA (As strand): | AAGCUUCGAACUUCCAUU
|
|
Wild sirna efficacy for wild allele: | 50
|
|
Wild allele (5'->3'): | UUCGAAGCUUGAAGGUAA
|
|
ASP-siRNA (As strand): | AAGCUUCGAACUUCGAUU
|
|
Wild sirna efficacy for mutant allele: | 90
|
|
Percentage discrimination for wild siRNA: | -40
|
| Concentration: | 1.2mIcrog |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | P61769 |
| Target sequence: | ATTGAAAAAGTGGAGCATTCAGACT |
| Genbank Accesion: | NM_004048.2 |
| Cell-line: | Epstein–Barr-virus-immortalized B-lymphocytes |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiFect |
| siRNA expression method: | Ambion |
| Post-transfection duration: | 48 hours |
| OMID ID: | NA |
| KEGG disease ID: | NA |
| Reference: | 16520945 |
