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siRNA Id: | aspsirna0923
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siRNA Name: | U6TAmut1
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Gene: | Torsin family 1, member A (TOR1A) |
Disease: | Early onset torsion dystonia (DYT1) |
Wild allele/target (5'->3'): | GAGUGGCUGAGGAGAUGACAUU
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Mutant allele/target (5'->3'): | GAGUGGCUGAG---AUGACAUU
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Target Mutation: | c.907_909delGAG,p.Glu303del
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Wild siRNA (As strand): | CUCACCGACUCCUCUACUGUAA
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ASP-siRNA (As strand): | CUCACCGACUC---UACUGUAA
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Mismatch position in siRNA: | NA |
Mutant allele (5'->3'): | GAGUGGCUGAG---AUGACAUU
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ASP-siRNA (As strand): | CUCACCGACUC---UACUGUAA
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ASP-siRNA efficacy for mutant allele: | 92
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Wild allele (5'->3'): | GAGUGGCUGAGGAGAUGACAUU
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ASP-siRNA (As strand): | CUCACCGACUC---UACUGUAA
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ASP-siRNA efficacy for wild allele: | 58
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Percentage discrimination for ASP-siRNA: | 34
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Wild allele (5'->3'): | GAGUGGCUGAGGAGAUGACAUU
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Wild siRNA (As strand): | CUCACCGACUCCUCUACUGUAA
|
Wild sirna efficacy for wild allele: | 40
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Wild allele (5'->3'): | GAGUGGCUGAGGAGAUGACAUU
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ASP-siRNA (As strand): | CUCACCGACUC---UACUGUAA
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Wild sirna efficacy for mutant allele: | 12
|
Percentage discrimination for wild siRNA: | 28
|
Concentration: | 100nM |
Orientation: | Double strand |
Mismatch in: | siRNA |
UniProt ID: | O14656 |
Target sequence: | AAGCAGAGTGGCTGAGGAGATGACATTTTTCCCCAAA |
Genbank Accesion: | NM_000113.2 |
Cell-line: | Cos-7 |
Experimental technique used: | Western blot |
Transfection method: | Lipofectamine Plus |
siRNA expression method: | Chemically synthesized |
Post-transfection duration: | 48 hours |
OMID ID: | 128100 |
KEGG disease ID: | H01255 |
Reference: | 16280588 |