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siRNA Id: | aspsirna0927
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siRNA Name: | U6TAmut5
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| Gene: | Torsin family 1, member A (TOR1A) |
| Disease: | Early onset torsion dystonia (DYT1) |
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Wild allele/target (5'->3'): | CAGAGUGGCUGAGGAGAUGACA
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Mutant allele/target (5'->3'): | CAGAGUGGCUGAG---AUGACA
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Target Mutation: | c.907_909delGAG,p.Glu303del
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Wild siRNA (As strand): | GUCUCACCGACUCCUCUACUGU
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ASP-siRNA (As strand): | GUCUCACCGACUC---UACUGU
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Mismatch position in siRNA: | NA |
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Mutant allele (5'->3'): | CAGAGUGGCUGAG---AUGACA
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ASP-siRNA (As strand): | GUCUCACCGACUC---UACUGU
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ASP-siRNA efficacy for mutant allele: | 95
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Wild allele (5'->3'): | CAGAGUGGCUGAGGAGAUGACA
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ASP-siRNA (As strand): | GUCUCACCGACUC---UACUGU
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ASP-siRNA efficacy for wild allele: | 15
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Percentage discrimination for ASP-siRNA: | 80
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Wild allele (5'->3'): | CAGAGUGGCUGAGGAGAUGACA
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Wild siRNA (As strand): | GUCUCACCGACUCCUCUACUGU
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Wild sirna efficacy for wild allele: | 40
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Wild allele (5'->3'): | CAGAGUGGCUGAGGAGAUGACA
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ASP-siRNA (As strand): | GUCUCACCGACUC---UACUGU
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Wild sirna efficacy for mutant allele: | 12
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Percentage discrimination for wild siRNA: | 28
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| Concentration: | 100nM |
| Orientation: | Double strand |
| Mismatch in: | siRNA |
| UniProt ID: | O14656 |
| Target sequence: | AAGCAGAGTGGCTGAGGAGATGACATTTTTCCCCAAA |
| Genbank Accesion: | NM_000113.2 |
| Cell-line: | Cos-7 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 48 hours |
| OMID ID: | 128100 |
| KEGG disease ID: | H01255 |
| Reference: | 16280588 |
